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Human identification and sex determination of dental pulp, bone marrow and blood stains with a recombinant DNA probe.

作者信息

Yokoi T, Aoki Y, Sagisaka K

机构信息

Department of Forensic Medicine, Tohoku University School of Medicine, Sendai, Japan.

出版信息

Z Rechtsmed. 1989;102(5):323-30. doi: 10.1007/BF00200221.

DOI:10.1007/BF00200221
PMID:2728626
Abstract

Recombinant DNA hybridizing specifically to a 300 nucleotide repeat DNA sequence (BLUR8) of human specificity and to human repeat DNA sequence (pHY10) on the Y chromosome was used for human identification and sex determination of degraded DNA samples of blood stains, dental pulp, and bone marrow. This radioactive technique enabled reliable and sensitive human and sex determination from blood stains that were more than 80 years old. Less than 1 piece of 0.5 cm length thread of blood stain was enough for both tests. DNA from relatively fresh dental pulp and bone marrow was clearly identified. The human identification test, which could recognize up to 0.3 ng DNA correctly, was 3 to 5 times more sensitive than the sex determination test.

摘要

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本文引用的文献

1
Regional assignment of a 2.1-kb repetitive sequence to the distal part of the human Y heterochromatin.一个2.1千碱基重复序列在人类Y异染色质远端部分的区域定位。
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Nihon Hoigaku Zasshi. 1983 Apr;37(2):127-32.
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Nihon Hoigaku Zasshi. 1983 Apr;37(2):84-7.
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J Mol Biol. 1981 Sep 5;151(1):17-33. doi: 10.1016/0022-2836(81)90219-9.
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Molecular cloning of Ancient Egyptian mummy DNA.古埃及木乃伊DNA的分子克隆
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Forensic application of DNA 'fingerprints'.DNA“指纹”的法医学应用。
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A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides.
Nucleic Acids Res. 1986 Oct 10;14(19):7569-80. doi: 10.1093/nar/14.19.7569.
8
Human blood stain identification and sex determination in dried blood stains using recombinant DNA techniques.利用重组DNA技术对干血渍进行人类血迹鉴定和性别判定。
Forensic Sci Int. 1986 Jul 31;31(4):267-72. doi: 10.1016/0379-0738(86)90166-0.
9
Overproduction of the protein product of a nonselected foreign gene carried by an adenovirus vector.腺病毒载体携带的非选择外源基因的蛋白质产物产生过量。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3567-71. doi: 10.1073/pnas.82.11.3567.
10
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Forensic Sci Int. 1989 Apr-May;41(1-2):117-24. doi: 10.1016/0379-0738(89)90243-0.