School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Gandhinagar 382007, Gujarat, India; Biotechnology Department, Kadi Sarva Vishwavidyalaya, Sector-15, Gandhinagar 382015, Gujarat, India.
Department of Microbiology, M. G. Science Institute, Ahmedabad 380009, Gujarat, India.
Bioresour Technol. 2016 Dec;221:510-516. doi: 10.1016/j.biortech.2016.09.080. Epub 2016 Sep 20.
The present research work was undertaken with a mandate to study carbon spectrum utilization and structural characterization of biosurfactant produced by indigenous Pseudomonas aeruginosa NCIM 5514, which showed unique properties to utilize a large number of carbon sources effectively for production of biosurfactant, although glucose was the best carbon substrate. In Bushnell-Hass medium supplemented with glucose (1%, w/v), 3.178±0.071g/l biosurfactant was produced by this isolate in 96h. The biosurfactant produced showed surface tension and emulsification activity values from 29.14±0.05 to 62.29±0.13mN/m and 88.50±1.96 to 15.40±0.91%, respectively. Toluene showed highest emulsification activity followed by kerosene. However, kerosene exhibited emulsion stability for 30days. Biosurfactant was characterized as a mixture of di-rhamnolipid (Rha-Rha-C-C) and mono-rhamnolipid (Rha-C-C) by FTIR, ESI-MS and LC-MS techniques. High biosurfactant yield opens up doors for the isolate to find utility in various industries.
本研究工作的任务是研究土著假单胞菌 NCIM 5514 产生的生物表面活性剂的碳谱利用和结构特征，尽管葡萄糖是最佳的碳源，但该菌具有独特的特性，能够有效地利用大量碳源生产生物表面活性剂。在补充有葡萄糖（1%，w/v）的 Bushnell-Hass 培养基中，该分离株在 96 小时内产生了 3.178±0.071g/l 的生物表面活性剂。所产生的生物表面活性剂的表面张力和乳化活性值分别为 29.14±0.05 至 62.29±0.13mN/m 和 88.50±1.96 至 15.40±0.91%。甲苯表现出最高的乳化活性，其次是煤油。然而，煤油表现出 30 天的乳液稳定性。生物表面活性剂通过 FTIR、ESI-MS 和 LC-MS 技术被表征为二鼠李糖脂（Rha-Rha-C-C）和单鼠李糖脂（Rha-C-C）的混合物。高生物表面活性剂产量为该分离株在各个行业中的应用开辟了道路。
