Ahmad Mahmoud Al, Panicker Neena G, Rizvi Tahir A, Mustafa Farah
Department of Electrical Engineering, College of Engineering, United Arab Emirates University, Al Ain, UAE.
Zayed Bin Sultan Center for Health Sciences Division United Arab Emirates University, Al Ain, UAE.
Sci Rep. 2016 Sep 28;6:34016. doi: 10.1038/srep34016.
High speed sequential identification of the building blocks of DNA, (deoxyribonucleotides or nucleotides for short) without labeling or processing in long reads of DNA is the need of the hour. This can be accomplished through exploiting their unique electrical properties. In this study, the four different types of nucleotides that constitute a DNA molecule were suspended in a buffer followed by performing several types of electrical measurements. These electrical parameters were then used to quantify the suspended DNA nucleotides. Thus, we present a purely electrical counting scheme based on the semiconductor theory that allows one to determine the number of nucleotides in a solution by measuring their capacitance-voltage dependency. The nucleotide count was observed to be similar to the multiplication of the corresponding dopant concentration and debye volume after de-embedding the buffer contribution. The presented approach allows for a fast and label-free quantification of single and mixed nucleotides in a solution.
在不进行DNA长读段标记或处理的情况下，高速连续识别DNA的组成单元（脱氧核糖核苷酸，简称核苷酸）是当下的迫切需求。这可以通过利用它们独特的电学性质来实现。在本研究中，构成DNA分子的四种不同类型的核苷酸悬浮在缓冲液中，随后进行了几种类型的电学测量。然后利用这些电学参数对悬浮的DNA核苷酸进行定量。因此，我们提出了一种基于半导体理论的纯电学计数方案，该方案允许通过测量核苷酸的电容-电压依赖性来确定溶液中核苷酸的数量。在去除缓冲液贡献后，观察到核苷酸计数与相应掺杂剂浓度和德拜体积的乘积相似。所提出的方法允许对溶液中的单核苷酸和混合核苷酸进行快速且无标记的定量。
