Ishidou Yasuhiro, Matsuyama Kanehiro, Matsuura Eiji, Setoguchi Takao, Nagano Satoshi, Kakoi Hironori, Hirotsu Masataka, Kawamura Ichiro, Yamamoto Takuya, Komiya Setsuro
Departments of Medical Joint Materials, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, 890-8520, Japan.
Departments of Neurology and Geriatrics, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Cell Tissue Bank. 2016 Dec;17(4):555-560. doi: 10.1007/s10561-016-9586-1. Epub 2016 Sep 27.
Allograft bone is a widely used as a convenient tool for reconstructing massive bone defects in orthopedic surgery. However, allografts are associated with the risk of viral disease transmission. One of the viruses transmitted in this manner is human T-lymphotropic virus type 1 (HTLV-1), which is found worldwide but is unevenly distributed. The southwestern parts of Japan are a highly endemic for HTLV-1. We investigated the HTLV-1 seroprevalence in candidate allograft donors at the regional bone bank in Kagoshima, Japan during its first 5 years of service. Between 2008 and 2012, we collected 282 femoral heads at the Kagoshima regional bone bank from living donors with osteoarthritis of the hip joint. Among the 282 candidate donors, 32 donors (11.3 %) were seropositive for anti-HTLV-1 antibody; notably, this prevalence is higher than that reported for blood donors in this area. Additionally, to determine if HTLV-1 genes are detectable after processing, we examined the bone marrow of the femoral heads from seropositive donors by conducting PCR assays. Our results confirm the existence of viral genes following the heat treatment processing of the femoral heads. Therefore, it is important to inactivate a virus completely by heat-treatment. Together, our findings highlight the importance of HTLV-1 screening at bone banks, particularly in HTLV-1-endemic areas such as southwest Japan.
同种异体骨是骨科手术中广泛用于重建大块骨缺损的便捷工具。然而，同种异体骨存在病毒疾病传播风险。以这种方式传播的病毒之一是1型人类嗜T淋巴细胞病毒（HTLV-1），该病毒在全球范围内均有发现，但分布不均。日本西南部是HTLV-1的高流行区。我们调查了日本鹿儿岛地区骨库在其服务的头5年中候选同种异体骨供体的HTLV-1血清阳性率。2008年至2012年期间，我们在鹿儿岛地区骨库从患有髋关节骨关节炎的活体供体处收集了282个股骨头。在这282名候选供体中，32名供体（11.3%）抗HTLV-1抗体血清呈阳性；值得注意的是，这一流行率高于该地区献血者的报告流行率。此外，为了确定在处理后是否能检测到HTLV-1基因，我们通过进行PCR检测对血清阳性供体的股骨髓进行了检查。我们的结果证实了股骨头热处理后病毒基因的存在。因此，通过热处理完全灭活病毒很重要。总之，我们的研究结果突出了在骨库进行HTLV-1筛查的重要性，尤其是在日本西南部等HTLV-1流行地区。
