嗜热自养甲烷杆菌δH甲基辅酶M甲基还原酶系统的A3组分：分解为两个组分。
Component A3 of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H: resolution into two components.
作者信息
Rouvière P E, Wolfe R S
机构信息
Department of Microbiology, University of Illinois, Urbana 61801.
出版信息
J Bacteriol. 1989 Sep;171(9):4556-62. doi: 10.1128/jb.171.9.4556-4562.1989.
Abstract
Component A3 of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum (strain delta H) has been resolved into two fractions. One, named component A3a, was defined as the fraction required along with components A2 and C to produce methane from 2-(methylthio)ethanesulfonate when titanium(III) citrate was used as the sole source of electrons. The second one, named component A3b, was required when H2 and 7-mercapto-N-heptanoyl-O-phospho-L-threonine were provided as the dual source of electrons. Component A3a was a large iron-sulfur protein aggregate (Mr 500,000) and is most likely involved in providing electrons at a low potential for the reductive activation of component C.
摘要
嗜热自养甲烷杆菌（菌株δH）的甲基辅酶M甲基还原酶系统的组分A3已被分离成两个部分。其中一个部分命名为组分A3a，被定义为当柠檬酸钛（III）用作唯一电子源时，与组分A2和C一起从2-（甲硫基）乙磺酸盐产生甲烷所需的部分。第二个部分命名为组分A3b，当提供H2和7-巯基-N-庚酰基-O-磷酸-L-苏氨酸作为双电子源时需要该部分。组分A3a是一种大的铁硫蛋白聚集体（Mr 500,000），最有可能参与为组分C的还原活化提供低电位电子。
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