甲烷杆菌甲基辅酶M甲基还原酶系统的组分A：分解为四个组分。
Component A of the methyl coenzyme M methylreductase system of Methanobacterium: resolution into four components.
作者信息
Nagle D P, Wolfe R S
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2151-5. doi: 10.1073/pnas.80.8.2151.
Abstract
Component A, the oxygen-sensitive protein fraction of the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum, has been stabilized and resolved into three protein fractions and one cofactor that are required to reconstitute component A activity. Component A1 is oxygen-stable and contains hydrogen-dependent deazaflavin (coenzyme F420)-reducing activity. Component A2 is acidic; components A2 and A3 are oxygen sensitive. The specific functions of each component in methyl group reduction are unknown. Resolution of component A revealed a new cofactor requirement of the methylreductase system for FAD. Hydrogen-dependent reduction of methyl coenzyme M to methane and coenzyme M, the terminal step of CO2 reduction by methanogenic bacteria, requires protein components A1, A2, A3, and C in addition to component B, FAD, ATP, and Mg2+.
摘要
组分A是嗜热自养甲烷杆菌甲基辅酶M甲基还原酶系统中对氧敏感的蛋白质部分，现已得到稳定，并分解为三个蛋白质部分和一个辅因子，这些是重建组分A活性所必需的。组分A1对氧稳定，具有依赖氢的脱氮黄素（辅酶F420）还原活性。组分A2呈酸性；组分A2和A3对氧敏感。各组分在甲基还原中的具体功能尚不清楚。组分A的分解揭示了甲基还原酶系统对FAD有新的辅因子需求。嗜甲烷菌将二氧化碳还原的最后一步，即依赖氢将甲基辅酶M还原为甲烷和辅酶M，除了组分B、FAD、ATP和Mg2+外，还需要蛋白质组分A1、A2、A3和C。
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