嗜热自养甲烷杆菌甲基辅酶M甲基还原酶系统中组分B（7-巯基庚酰苏氨酸磷酸）的结构
Structure of component B (7-mercaptoheptanoylthreonine phosphate) of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum.
作者信息
Noll K M, Rinehart K L, Tanner R S, Wolfe R S
出版信息
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4238-42. doi: 10.1073/pnas.83.12.4238.
Abstract
Component B, the heat-stable low-molecular-weight cofactor required for methane production by dialyzed cell-free extracts of Methanobacterium thermoautotrophicum, has been purified to homogeneity and its structure assigned. Results of low-resolution fast-atom-bombardment and field-desorption mass spectrometry indicated a molecular weight of 419, and high-resolution fast-atom-bombardment mass spectrometry agreed with the molecular formula C13H26NO8PS2. Evidence from fast-atom-bombardment and field-desorption mass spectrometry and 360-MHz 1H NMR in deuterium oxide argued that the compound was isolated as a mixed disulfide with 2-mercaptoethanol; so the proposed elemental formula of the free acid, free thiol would be C11H22NO7PS (molecular weight, 343). The proposed structure for an active form of the coenzyme is 7-mercaptoheptanoylthreonine phosphate.
摘要
成分B是嗜热自养甲烷杆菌透析后的无细胞提取物产生甲烷所必需的热稳定低分子量辅因子，已被纯化至同质并确定了其结构。低分辨率快原子轰击和场解吸质谱结果表明分子量为419，高分辨率快原子轰击质谱与分子式C13H26NO8PS2相符。快原子轰击和场解吸质谱以及重水中的360兆赫1H核磁共振证据表明，该化合物是以与2-巯基乙醇形成的混合二硫化物形式分离出来的；因此，游离酸、游离硫醇的建议分子式为C11H22NO7PS（分子量343）。辅酶活性形式的建议结构为7-巯基庚酰苏氨酸磷酸酯。
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Structure of component B (7-mercaptoheptanoylthreonine phosphate) of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum.嗜热自养甲烷杆菌甲基辅酶M甲基还原酶系统中组分B（7-巯基庚酰苏氨酸磷酸）的结构
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