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包含来自短短芽孢杆菌的短杆菌酪肽合成酶1和2基因的基因簇：操纵子的证据。

Gene cluster containing the genes for tyrocidine synthetases 1 and 2 from Bacillus brevis: evidence for an operon.

作者信息

Mittenhuber G, Weckermann R, Marahiel M A

机构信息

Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Federal Republic of Germany.

出版信息

J Bacteriol. 1989 Sep;171(9):4881-7. doi: 10.1128/jb.171.9.4881-4887.1989.

DOI:10.1128/jb.171.9.4881-4887.1989
PMID:2768192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210293/
Abstract

From a genomic library of the tyrocidine producer Bacillus brevis ATCC 8185 constructed in the bacteriophage vector EMBL3, a recombinant phage which contains the structural genes coding for tyrocidine synthetases 1 and 2, TycA and TycB, was identified. The location of the tycA gene within the 16-kilobase insert of this clone, EMBL25-1, was mapped by hybridization studies by using the previously isolated tycA DNA as a probe. Restriction analyses, the construction of subclones, and the analysis of proteins encoded by the subclones located the tycB gene at the 3' end of the tycA gene and revealed that the two genes are transcribed in the same direction. Nuclease S1 protection studies and DNA sequencing studies of the intergenic region indicated that tycA and tycB are separated by a 94-base-pair noncoding region and suggested that these genes are organized as an operon.

摘要

从用噬菌体载体EMBL3构建的短杆菌酪肽产生菌短短芽孢杆菌ATCC 8185的基因组文库中，鉴定出一个重组噬菌体，它含有编码酪肽合成酶1和2（TycA和TycB）的结构基因。通过使用先前分离的tycA DNA作为探针进行杂交研究，确定了该克隆EMBL25 - 1的16千碱基插入片段内tycA基因的位置。限制性分析、亚克隆构建以及亚克隆编码蛋白质的分析将tycB基因定位在tycA基因的3'端，并表明这两个基因以相同方向转录。核酸酶S1保护研究和基因间区域的DNA测序研究表明，tycA和tycB被一个94个碱基对的非编码区域隔开，并提示这些基因被组织成一个操纵子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/d9021fd27116/jbacter00175-0369-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/964a1abba6cc/jbacter00175-0367-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/bc1233c952e2/jbacter00175-0367-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/517bd5457275/jbacter00175-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/8ebad1aebc69/jbacter00175-0368-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/d9021fd27116/jbacter00175-0369-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/964a1abba6cc/jbacter00175-0367-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/bc1233c952e2/jbacter00175-0367-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/517bd5457275/jbacter00175-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/8ebad1aebc69/jbacter00175-0368-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6979/210293/d9021fd27116/jbacter00175-0369-a.jpg

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