Morris M E, Jinks-Robertson S
Department of Biology, Emory University, Atlanta, GA 30322.
Gene. 1991 Feb 1;98(1):141-5. doi: 10.1016/0378-1119(91)90117-t.
The Saccharomyces cerevisiae LYS2 gene, which encodes alpha-aminoadipate reductase, an essential enzyme in the yeast lysine biosynthetic pathway, has been sequenced. A large open reading frame (ORF) has been identified which can specify a 1392-amino acid protein with a deduced Mr of 155,344. A DNA database search using the translated LYS2 ORF as a probe has revealed significant aa sequence homology to the Bacillus brevis enzyme tyrocidine synthetase 1.
编码α-氨基己二酸还原酶（酵母赖氨酸生物合成途径中的一种必需酶）的酿酒酵母LYS2基因已被测序。已鉴定出一个大的开放阅读框（ORF），它可以编码一种1392个氨基酸的蛋白质，推导的分子量为155,344。使用翻译后的LYS2 ORF作为探针进行的DNA数据库搜索显示，与短短芽孢杆菌的酶短杆菌酪肽合成酶1有显著的氨基酸序列同源性。
