Hadjilouka Agni, Polychronopoulou Melissanthi, Paramithiotis Spiros, Tzamalis Periklis, Drosinos Eleftherios H
Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, GR-118 55 Athens, Greece.
Microorganisms. 2015 Sep 9;3(3):535-50. doi: 10.3390/microorganisms3030535.
The aim of the present study was to examine the effect of lemongrass essential oil vapors on the dynamics of surface microbiota and L. monocytogenes growth on rocket and melon under different packaging conditions and storage temperature. For that purpose, rocket and melon were placed on Expanded Polystyrene (EPS) trays, sprayed with L. monocytogenes to a population of 4.5-5.0 log CFU·g(-1), packaged using microperforated Oriented Polypropylene (OPP) film in either air or Microperforated Active Modified Atmosphere (MAMA) (initial atmosphere 5% O₂, 10% CO₂) including a Whatman paper containing the essential oil, without contact with the product, and stored at 0, 5, 10, and 15 °C. Application of lemongrass exhibited a bactericidal effect on enterococci and a fungistatic effect on yeast-mould populations but only during air storage of rocket. The former took place at all temperatures and the latter only at 10 and 15 °C. No effect on shelf life of both products was recorded. However, an important effect on the sensorial properties was observed; during the first 4-5 days of storage both products were organoleptically unacceptable. Regarding MAMA packaging, it affected only Pseudomonas spp. population resulting in a reduction of 1-2 log CFU·g(-1) in both products.
本研究的目的是考察柠檬草精油蒸汽在不同包装条件和储存温度下对芝麻菜和甜瓜表面微生物群落动态以及单核细胞增生李斯特菌生长的影响。为此，将芝麻菜和甜瓜放在聚苯乙烯泡沫塑料（EPS）托盘上，喷洒单核细胞增生李斯特菌使其菌量达到4.5 - 5.0 log CFU·g⁻¹，使用微孔定向聚丙烯（OPP）薄膜在空气或微孔活性气调包装（MAMA）（初始气体成分为5% O₂、10% CO₂）中进行包装，其中MAMA包装包含一张含有精油的沃特曼滤纸，滤纸不与产品接触，然后分别在0、5、10和15℃下储存。柠檬草的应用对肠球菌表现出杀菌作用，对酵母 - 霉菌菌群表现出抑菌作用，但仅在芝麻菜空气储存期间如此。前者在所有温度下均发生，后者仅在10℃和15℃下发生。未记录到对两种产品货架期的影响。然而，观察到对感官特性有重要影响；在储存的前4 - 5天，两种产品在感官上都不可接受。关于MAMA包装，它仅影响假单胞菌属菌群，导致两种产品中的菌量均减少1 - 2 log CFU·g⁻¹。
