Park Hong-Su, Lim Il-Soo, Kim Sang-Kyu, Kim Toh-Kyung, Park Choi-Kyu, Yeo Sang-Geon
College of Veterinary Medicine and Animal Diseases Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea.
Vaccine and Infectious Disease Organization, 120 Veterinary Road, Saskatoon, SK, S7N 5E3, Canada.
Virus Genes. 2017 Feb;53(1):111-116. doi: 10.1007/s11262-016-1393-z. Epub 2016 Sep 28.
Fowl adenovirus serotype 4 (FAdV-4) is the causative agent of hydropericardium syndrome (HPS), a highly pathogenic disease in poultry. In the present study, hexon, penton base, and fiber-2 genes encoding major capsid proteins were analyzed in four FAdV-4 isolates from HPS-affected chickens in Korea. Nucleotide sequences of the entire hexon (2811 bases), penton base (1578 bases), and fiber-2 (1425 bases) genes from the Korean isolates were 97.5-99.3, 99.1-99.7, and 95.5-99.0 % identical, respectively, to those of foreign FAdV-4 isolates. In the N-terminal tail region of fiber-2, the KRP motif predicted to be the nuclear localization signal was identified in the Korean isolates, whereas KRP/A was detected in other isolates. The VYPF motif in fiber-2, which is known to interact with the penton base, was present in the same region of all FAdV-4 isolates that were compared. Amino acid variations in fiber-2 for HPS and non-HPS isolates revealed that D219 and T300 were conserved among ten HPS isolates from five countries, including Korea. T380 in fiber-2, previously found in HPS isolates, corresponded to A380 in the Korean isolates, indicating that T380 is not relevant for increased virulence. Phylogenetic analysis showed that the four Korean FAdV-4 isolates were more related to MX-SHP95, a Mexican FAdV-4 isolate of HPS origin, than to FAdV-4 isolates of Indian and Chinese origin, suggesting that the genetic relationship among FAdV-4 isolates is independent of geographic distribution. The molecular features of these genes will provide valuable information for vaccine development against HPS in the future.
禽腺病毒4型（FAdV-4）是心包积水综合征（HPS）的病原体，这是一种在家禽中具有高度致病性的疾病。在本研究中，对来自韩国受HPS影响的鸡的4株FAdV-4分离株中编码主要衣壳蛋白的六邻体、五邻体基座和纤维-2基因进行了分析。韩国分离株的整个六邻体（2811个碱基）、五邻体基座（1578个碱基）和纤维-2（1425个碱基）基因的核苷酸序列与国外FAdV-4分离株的核苷酸序列分别有97.5-99.3%、99.1-99.7%和95.5-99.0%的同一性。在纤维-2的N端尾部区域，在韩国分离株中鉴定出预测为核定位信号的KRP基序，而在其他分离株中检测到KRP/A。纤维-2中已知与五邻体基座相互作用的VYPF基序存在于所有被比较的FAdV-4分离株的同一区域。HPS和非HPS分离株的纤维-2氨基酸变异表明，D219和T300在包括韩国在内的五个国家的10株HPS分离株中是保守的。先前在HPS分离株中发现的纤维-2中的T380在韩国分离株中对应于A380，表明T380与毒力增加无关。系统发育分析表明，4株韩国FAdV-4分离株与源自墨西哥的HPS分离株MX-SHP95的亲缘关系比与源自印度和中国的FAdV-4分离株更近，这表明FAdV-分离株之间的遗传关系与地理分布无关。这些基因的分子特征将为未来开发针对HPS的疫苗提供有价值的信息。
