Huang Sheng-Zhuo, Kong Fan-Dong, Ma Qing-Yun, Guo Zhi-Kai, Zhou Li-Man, Wang Qi, Dai Hao-Fu, Zhao You-Xing
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences , Haikou 571101, People's Republic of China.
J Nat Prod. 2016 Oct 28;79(10):2599-2605. doi: 10.1021/acs.jnatprod.6b00528. Epub 2016 Sep 29.
Eight new alkaloids, 3β-n-butylstemonamine (1), 8-oxo-3β-n-butylstemonamine (2), 3-n-butylneostemonine (3), 10-epi-3-n-butylneostemonine (4), 8-oxo-oxymaistemonine (5) protostemonine N-oxide (6), (19S)-hydroxy-21-methoxystemofoline (7), and parvistemonine A (8), were isolated from the roots of Stemona parviflora, together with 17 known alkaloids. The structures of the new alkaloids were elucidated based on a comprehensive spectroscopic data analysis. The absolute configurations of 1-4 were determined by the ECD exciton chirality method and quantum ECD calculations. Protostemonine (10) and stemofoline (12) showed strong nematicidal activity against Panagrellus redivevus, with IC values of 0.10 and 0.46 μM, respectively.
从小花百部的根部分离得到8个新生物碱，分别为3β-正丁基百部碱（1）、8-氧代-3β-正丁基百部碱（2）、3-正丁基新百部碱（3）、10-表-3-正丁基新百部碱（4）、8-氧代氧化迈百部碱（5）、原百部碱N-氧化物（6）、（19S）-羟基-21-甲氧基百部叶碱（7）和小花百部碱A（8），同时还分离得到17个已知生物碱。通过全面的光谱数据分析阐明了新生物碱的结构。利用ECD激子手性方法和量子ECD计算确定了1-4的绝对构型。原百部碱（10）和百部叶碱（12）对小杆线虫表现出较强的杀线虫活性，IC值分别为0.10和0.46 μM。
