Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, China.
Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, China.
Phytochemistry. 2019 Mar;159:208-215. doi: 10.1016/j.phytochem.2018.12.021. Epub 2019 Jan 8.
Twelve undescribed compounds including six phenanthrene derivatives (parviphenanthrines A-F), two stilbene derivatives (parvistilbines A-B), three esters (parviesters A-C), and one sesquiterpenoid (parvidiol A) were isolated from the roots of Stemona parviflora, together with twenty-two known ones. The structures of the undescribed compounds were elucidated based on the analyses of their spectroscopic data. The absolute configuration of parviphenanthrine A was determined by the quantum ECD calculations. Parviphenanthrines A and E, stemanthrene A, stilbostenin E, 4-hydroxy-benzenepropanol-α-benzoate, and (E)-4-hydroxycinnamic acid methyl ester showed nematocidal activity against Meloidogyne incognita with IC values of 14.02 ± 0.32, 2.51 ± 0.13, 17.10 ± 0.65, 2.05 ± 0.07, 4.22 ± 0.31, and 1.07 ± 0.05 μM, respectively.
从柔弱百部中分离得到 12 个化合物，包括 6 个菲衍生物（parviphenanthrines A-F）、2 个二苯乙烯衍生物（parvistilbines A-B）、3 个酯（parviesters A-C）和 1 个倍半萜（parvidiol A），以及 22 个已知化合物。根据光谱数据分析阐明了这些未知化合物的结构。通过量子ECD 计算确定了 parviphenanthrine A 的绝对构型。Parviphenanthrines A 和 E、stemanthrene A、stilbostenin E、4-羟基苯丙酸-α-苯甲酸酯和(E)-4-羟基肉桂酸甲酯对南方根结线虫具有杀线虫活性，IC值分别为 14.02 ± 0.32、2.51 ± 0.13、17.10 ± 0.65、2.05 ± 0.07、4.22 ± 0.31 和 1.07 ± 0.05 μM。
