Karlíková R, Široká J, Jahn P, Friedecký D, Gardlo A, Janečková H, Hrdinová F, Drábková Z, Adam T
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic.
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic.
Vet J. 2016 Oct;216:125-32. doi: 10.1016/j.tvjl.2016.07.015. Epub 2016 Jul 29.
Atypical myopathy (AM) is a potentially fatal disease of grazing horses. It is reportedly caused by the ingestion of sycamore seeds containing toxic hypoglycin A. In order to study metabolic changes, serum and urine samples from nine horses with atypical myopathy and 12 control samples from clinically healthy horses were collected and then analysed using a high-performance liquid chromatography coupled with tandem mass spectrometry; serum metabolic profiles as the disease progressed were also studied. Metabolic data were evaluated using unsupervised and supervised multivariate analyses. Significant differences were demonstrated in the concentrations of various glycine conjugates and acylcarnitines (C2-C26). Moreover, the concentrations of purine and pyrimidine metabolites, vitamins and their degradation products (riboflavin, trigonelline, pyridoxate, pantothenate), and selected organic and amino acids (aspartate, leucine, 2-oxoglutarate, etc.) were altered in horses with AM. These results represent a global view of altered metabolism in horses with atypical myopathy.
非典型肌病（AM）是一种可能导致放牧马匹死亡的疾病。据报道，它是由摄入含有毒性次甘氨酸A的梧桐种子引起的。为了研究代谢变化，收集了9匹患有非典型肌病的马的血清和尿液样本以及12份临床健康马的对照样本，然后使用高效液相色谱-串联质谱法进行分析；还研究了疾病进展过程中的血清代谢谱。代谢数据通过无监督和有监督的多变量分析进行评估。各种甘氨酸共轭物和酰基肉碱（C2 - C26）的浓度存在显著差异。此外，患有非典型肌病的马体内嘌呤和嘧啶代谢物、维生素及其降解产物（核黄素、胡芦巴碱、吡哆酸、泛酸盐）以及选定的有机和氨基酸（天冬氨酸、亮氨酸、2-氧代戊二酸等）的浓度发生了变化。这些结果代表了非典型肌病马匹代谢改变的整体情况。
