甲醇和甲酸对大鼠视网膜功能的中毒作用。

The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function.

作者信息

Liu Dong-Mei, Zhou Shu, Chen Jie-Min, Peng Shu-Ya, Xia Wen-Tao

机构信息

Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, China; Institute of Shanghai Huayi Forensic Science, Shanghai 200335, China.

Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, China.

出版信息

J Ophthalmol. 2016;2016:4087096. doi: 10.1155/2016/4087096. Epub 2016 Sep 4.

DOI:10.1155/2016/4087096

PMID:27688906

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5027045/

Abstract

UNLABELLED

Objective. To explore the potential effects of methanol and its metabolite, formic acid, on rat retina function. Methods. Sprague-Dawley rats were divided into 3- and 7-day groups and a control. Experimental groups were given methanol and the control group were provided saline by gavage. Retinal function of each group was assessed by electroretinogram. Concentrations of methanol and formic acid were detected by GC/HS and HPLC, respectively. Results. The a and b amplitudes of methanol treated groups decreased and latent periods delayed in scotopic and photopic ERG recordings. The summed amplitudes of oscillatory potentials (OPs) of groups B and C decreased and the elapsed time delayed. The amplitudes of OS1, OS3, OS4, and OS5 of group B and OS3, OS4, and OS5 of group C decreased compared with the control group. The IPI1 of group B and IPI1-4 of group C were broader compared with the control group and the IPI1-4 and ET of group B were broader than group C.

CONCLUSIONS

Both of scotopic and photopic retinal functions were impaired by methanol poisoning, and impairment was more serious in the 7-day than in the 3-day group. OPs, especially later OPs and IPI2, were more sensitive to methanol intoxication than other eletroretinogram subcomponents.

摘要

未标记

目的。探讨甲醇及其代谢产物甲酸对大鼠视网膜功能的潜在影响。方法。将Sprague-Dawley大鼠分为3天组、7天组和对照组。给实验组大鼠灌胃甲醇，给对照组大鼠灌胃生理盐水。通过视网膜电图评估每组大鼠的视网膜功能。分别采用气相色谱/顶空进样法（GC/HS）和高效液相色谱法（HPLC）检测甲醇和甲酸的浓度。结果。在暗视和明视视网膜电图记录中，甲醇处理组的a波和b波振幅降低，潜伏期延长。B组和C组的振荡电位（OPs）总和振幅降低，持续时间延长。与对照组相比，B组的OS1、OS3、OS4和OS5振幅以及C组的OS3、OS4和OS5振幅降低。与对照组相比，B组的IPI1和C组的IPI1-4变宽，且B组的IPI1-4和ET比C组更宽。