Román Elvira, Prieto Daniel, Martin Ry, Correia Inês, Mesa Arango Ana Cecilia, Alonso-Monge Rebeca, Zaragoza Oscar, Pla Jesús
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal s/n, E-28040 Madrid, Spain.
Group of Investigative Dermatology, University of Antioquía, Medellín, Colombia.
Future Microbiol. 2016 Oct;11:1279-1297. doi: 10.2217/fmb-2016-0067. Epub 2016 Oct 3.
To investigate the role of Cat1 overproduction in Candida albicans.
MATERIALS & METHODS: Strains overproducing the CAT1 gene were constructed.
Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen species generated by amphotericin B or ciclopirox olamine. CAT1 overproduction did not change the minimum inhibitory concentration of fungal cells to fungistatic or fungicidal azoles nor to amphotericin B although increased twofold the minimum inhibitory concentration to caspofungin. The role of Cat1 overproduction in virulence and colonization was also analyzed in mouse models.
The overproduction of Cat1 protects against oxidants, phagocytes and certain antifungals at subinhibitory concentration but does not increase virulence in a systemic infection mouse model.
研究白色念珠菌中Cat1过量表达的作用。
构建了过量表达CAT1基因的菌株。
发现过量表达CAT1的细胞对某些氧化剂和哺乳动物吞噬细胞更具抗性。它们还显示出两性霉素B或环吡酮胺产生的细胞内活性氧减少。尽管将卡泊芬净的最低抑菌浓度提高了两倍，但CAT1的过量表达并未改变真菌细胞对抑菌或杀菌唑类药物以及两性霉素B的最低抑菌浓度。还在小鼠模型中分析了Cat1过量表达在毒力和定植中的作用。
Cat1的过量表达可在亚抑菌浓度下抵御氧化剂、吞噬细胞和某些抗真菌药物，但在全身感染小鼠模型中不会增加毒力。
