Puig-Domingo M, Guerrero J M, Menéndez-Pelaez A, Reiter R J
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
J Endocrinol. 1989 Aug;122(2):553-6. doi: 10.1677/joe.0.1220553.
The response of type-II thyroxine 5'-deiodinase (5'-DII) to melatonin treatment was studied in the Syrian hamster. Male hamsters were treated for 15 days with a s.c. pellet containing melatonin, and 5'-DII activity in brown adipose tissue, anterior pituitary gland, Harderian gland and pineal gland was measured using a radioenzymatic technique. Melatonin-treated animals exhibited enhanced 5'-DII activity restricted to brown adipose tissue; the increase was threefold above the values measured in the control group. Serum concentrations of thyroid hormones were unaffected by melatonin treatment. We conclude that the stimulatory effect of melatonin on type-II thyroxine 5'-deiodination is specifically directed to the isoenzyme located in brown adipose tissue and is not accompanied by changes in serum thyroid hormones.
在叙利亚仓鼠中研究了II型甲状腺素5'-脱碘酶（5'-DII）对褪黑素治疗的反应。雄性仓鼠用含褪黑素的皮下植入丸治疗15天，使用放射酶法测量棕色脂肪组织、垂体前叶、哈德氏腺和松果体中的5'-DII活性。褪黑素治疗的动物在棕色脂肪组织中表现出5'-DII活性增强；增加幅度比对照组测量值高两倍。甲状腺激素的血清浓度不受褪黑素治疗的影响。我们得出结论，褪黑素对II型甲状腺素5'-脱碘的刺激作用特异性地针对位于棕色脂肪组织中的同工酶，且不伴有血清甲状腺激素的变化。
