Szcześniak Katarzyna A, Ciecierska Anna, Ostaszewski Piotr, Sadkowski Tomasz
Department of Physiological Sciences, Faculty of Veterinary Medicine,Warsaw University of Life Science - SGGW,Nowoursynowska 159, 02-776 Warsaw,Poland.
Br J Nutr. 2016 Oct;116(8):1315-1325. doi: 10.1017/S000711451600324X. Epub 2016 Oct 3.
β-Hydroxy-β-methylbutyrate (HMB) is a popular ergogenic aid used by human athletes and as a supplement to sport horses, because of its ability to aid muscle recovery, improve performance and body composition. Recent findings suggest that HMB may stimulate satellite cells and affect expressions of genes regulating skeletal muscle cell growth. Despite the scientific data showing benefits of HMB supplementation in horses, no previous study has explained the mechanism of action of HMB in this species. The aim of this study was to reveal the molecular background of HMB action on equine skeletal muscle by investigating the transcriptomic profile changes induced by HMB in equine satellite cells in vitro. Upon isolation from the semitendinosus muscle, equine satellite cells were cultured until the 2nd day of differentiation. Differentiating cells were incubated with HMB for 24 h. Total cellular RNA was isolated, amplified, labelled and hybridised to microarray slides. Microarray data validation was performed with real-time quantitative PCR. HMB induced differential expressions of 361 genes. Functional analysis revealed that the main biological processes influenced by HMB in equine satellite cells were related to muscle organ development, protein metabolism, energy homoeostasis and lipid metabolism. In conclusion, this study demonstrated for the first time that HMB has the potential to influence equine satellite cells by controlling global gene expression. Genes and biological processes targeted by HMB in equine satellite cells may support HMB utility in improving growth and regeneration of equine skeletal muscle; however, the overall role of HMB in horses remains equivocal and requires further proteomic, biochemical and pharmacokinetic studies.
β-羟基-β-甲基丁酸酯（HMB）是一种广受欢迎的促力剂，被人类运动员使用，并作为运动马匹的补充剂，因为它能够帮助肌肉恢复、提高运动表现和改善身体成分。最近的研究结果表明，HMB可能刺激卫星细胞，并影响调节骨骼肌细胞生长的基因表达。尽管有科学数据表明补充HMB对马匹有益，但此前尚无研究解释HMB在该物种中的作用机制。本研究的目的是通过研究体外培养的马卫星细胞中HMB诱导的转录组谱变化，揭示HMB对马骨骼肌作用的分子背景。从半腱肌分离后，将马卫星细胞培养至分化的第2天。将分化中的细胞与HMB孵育24小时。分离、扩增、标记总细胞RNA，并与微阵列玻片杂交。用实时定量PCR进行微阵列数据验证。HMB诱导了361个基因的差异表达。功能分析表明，HMB在马卫星细胞中影响的主要生物学过程与肌肉器官发育、蛋白质代谢、能量稳态和脂质代谢有关。总之，本研究首次证明HMB有可能通过控制全局基因表达来影响马卫星细胞。HMB在马卫星细胞中靶向的基因和生物学过程可能支持HMB在改善马骨骼肌生长和再生方面的效用；然而，HMB在马匹中的整体作用仍不明确，需要进一步的蛋白质组学、生物化学和药代动力学研究。
