He Yupeng, Nie Enguang, Li Chengming, Ye Qingfu, Wang Haiyan
Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang Province, Zhejiang University, Hangzhou 310029, China.
Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang Province, Zhejiang University, Hangzhou 310029, China.
Environ Pollut. 2017 Jan;220(Pt A):400-406. doi: 10.1016/j.envpol.2016.09.076. Epub 2016 Sep 29.
The increasing discharge of pharmaceuticals and personal care products (PPCPs) into the environment has generated serious public concern. The recent awareness of the environmental impact of this emerging class of pollutants and their potential adverse effects on human health have been documented in many reports. However, information regarding uptake and intracellular distribution of PPCPs in hydrophytes under hydroponic conditions, and potential human exposure is very limited. A laboratory experiment was conducted using C-labeled triclosan (TCS) to investigate uptake and distribution of TCS in six aquatic plants (water spinach, purple perilla, cress, penny grass, cane shoot, and rice), and the subcellular distribution of C-TCS was determined in these plants. The results showed that the uptake and removal rate of TCS from nutrient solution by hydrophytes followed the order of cress (96%) > water spinach (94%) > penny grass (87%) > cane shoot (84%) > purple perilla (78%) > rice (63%) at the end of incubation period (192 h). The range of C-TCS content in the roots was 94.3%-99.0% of the added C-TCS, and the concentrations in roots were 2-3 orders of magnitude greater than those in shoots. Furthermore, the subcellular fraction-concentration factor (3.6 × 10-2.6 × 10 mL g), concentration (0.58-4.47 μg g), and percentage (30%-61%) of C-TCS in organelles were found predominantly greater than those in cell walls and/or cytoplasm. These results indicate that for these plants, the roots are the primary storage for TCS, and within plant cells organelles are the major domains for TCS accumulation. These findings provide a better understanding of translocation and accumulation of TCS in aquatic plants at the cellular level, which is valuable for environmental and human health assessments of TCS.
药物和个人护理产品（PPCPs）向环境中的排放量不断增加，已引起公众的严重关注。近期许多报告都记录了这类新兴污染物对环境的影响及其对人类健康的潜在不利影响。然而，关于水培条件下水生植物对PPCPs的吸收和细胞内分布以及潜在的人体暴露的信息非常有限。进行了一项实验室实验，使用碳标记的三氯生（TCS）来研究TCS在六种水生植物（空心菜、紫苏、水芹、铜钱草、甘蔗和水稻）中的吸收和分布，并测定了这些植物中碳标记TCS的亚细胞分布。结果表明，在培养期结束时（192小时），水生植物从营养液中吸收和去除TCS的速率顺序为：水芹（96%）＞空心菜（94%）＞铜钱草（87%）＞甘蔗（84%）＞紫苏（78%）＞水稻（63%）。根部碳标记TCS的含量范围为添加碳标记TCS的94.3% - 99.0%，根部浓度比地上部分高2 - 3个数量级。此外，发现细胞器中碳标记TCS的亚细胞组分浓度因子（3.6×10 - 2.6×10 mL g）、浓度（0.58 - 4.47 μg g）和百分比（30% - 61%）主要大于细胞壁和/或细胞质中的。这些结果表明，对于这些植物，根部是TCS的主要储存部位，在植物细胞内，细胞器是TCS积累的主要区域。这些发现有助于在细胞水平上更好地理解TCS在水生植物中的转运和积累，这对于TCS的环境和人类健康评估具有重要价值。
