Forest Valérie, Leclerc Lara, Hochepied Jean-François, Trouvé Adeline, Sarry Gwendoline, Pourchez Jérémie
Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France; INSERM, U1059, F-42023 Saint Etienne, France; Université de Lyon, F-69000 Lyon, France.
Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France; INSERM, U1059, F-42023 Saint Etienne, France; Université de Lyon, F-69000 Lyon, France.
Toxicol In Vitro. 2017 Feb;38:136-141. doi: 10.1016/j.tiv.2016.09.022. Epub 2016 Sep 28.
Cerium oxides (CeO) nanoparticles, also referred to as nanoceria, are extensively used with a wide range of applications. However, their impact on human health and on the environment is not fully elucidated. The aim of this study was to investigate the influence of the CeO nanoparticles morphology on their in vitro toxicity. CeO nanoparticles of similar chemical composition and crystallinity were synthesized, only the shape varied (rods or octahedrons/cubes). Macrophages from the RAW264.7 cell line were exposed to these different samples and the toxicity was evaluated in terms of lactate dehydrogenase (LDH) release, Tumor Necrosis Factor alpha (TNF-α) production and reactive oxygen species (ROS) generation. Results showed no ROS production, whatever the nanoparticle shape. The LDH release and the TNF-α production were significantly and dose-dependently enhanced by rod-like nanoparticles, whereas they did not vary with cubic/octahedral nanoparticles. In conclusion, a strong impact of CeO nanoparticle morphology on their in vitro toxicity was clearly demonstrated, underscoring that nanoceria shape should be carefully taken in consideration, especially in a "safer by design" context.
氧化铈（CeO）纳米颗粒，也被称为纳米氧化铈，被广泛应用于众多领域。然而，它们对人类健康和环境的影响尚未完全阐明。本研究的目的是调查CeO纳米颗粒形态对其体外毒性的影响。合成了化学成分和结晶度相似的CeO纳米颗粒，仅形状不同（棒状或八面体/立方体）。将RAW264.7细胞系的巨噬细胞暴露于这些不同的样品中，并根据乳酸脱氢酶（LDH）释放、肿瘤坏死因子α（TNF-α）产生和活性氧（ROS）生成来评估毒性。结果表明，无论纳米颗粒形状如何，均未产生ROS。棒状纳米颗粒显著且剂量依赖性地增强了LDH释放和TNF-α产生，而立方/八面体纳米颗粒则无此变化。总之，CeO纳米颗粒形态对其体外毒性有显著影响，这突出表明在“设计更安全”的背景下，应特别谨慎地考虑纳米氧化铈的形状。
