Pavlides C, Winson J
Rockefeller University, New York, New York 10021.
J Neurosci. 1989 Aug;9(8):2907-18. doi: 10.1523/JNEUROSCI.09-08-02907.1989.
Rat hippocampal (CA1) complex spike "place cells" of freely behaving rats were recorded in pairs continuously during a series of waking (exploration and still-alert), drowsy (quiet-awake), and sleeping (slow-wave, pre-rapid-eye-movement and rapid-eye-movement sleep) behaviors. Pairs of units were selected that had nonoverlapping place fields. The rats were restricted from entering the place field of either cell overnight, and on the day of recording cells were exposed to their individual place fields independently and in a counterbalanced manner. Following exposure, recordings were made in the subsequent sleep episodes and the firing characteristics of both cells were analyzed. Following exposure, significant increases in the spiking activity of the exposed cell were observed in the subsequent sleeping states that were not evident in the unexposed cell. The increased activity was observed in the rate of firing (spikes/sec), the rate of occurrence of bursts with multiple spikes, as well as the number of bursts displaying short (2-4 msec) interspike intervals. The findings suggest that neuronal activity of hippocampal place cells in the awake states may influence the firing characteristics of these cells in subsequent sleep episodes. The increased firing rates along with the greater number of multiple spike bursts and the shorter interspike intervals within the burst, following exposure to a cell's place field, may represent possible information processing during sleep.
在一系列清醒（探索和静息警觉）、困倦（安静清醒）和睡眠（慢波睡眠、快速眼动睡眠前期和快速眼动睡眠）行为期间，连续成对记录自由活动大鼠的海马体（CA1）复合锋电位“位置细胞”。选择具有不重叠位置野的成对神经元。大鼠被限制在夜间进入任何一个细胞的位置野，在记录当天，细胞分别以平衡的方式独立暴露于它们各自的位置野。暴露后，在随后的睡眠阶段进行记录，并分析两个细胞的放电特征。暴露后，在随后的睡眠状态中观察到暴露细胞的放电活动显著增加，而未暴露细胞则不明显。在放电频率（每秒尖峰数）、多峰爆发的发生率以及显示短（2 - 4毫秒）峰间间隔的爆发次数方面都观察到了活动增加。这些发现表明，清醒状态下海马体位置细胞的神经元活动可能会影响这些细胞在随后睡眠阶段的放电特征。在暴露于细胞的位置野后，放电频率增加，多峰爆发次数增多，爆发内峰间间隔更短，这可能代表了睡眠期间可能的信息处理过程。
