Research center for the Physics of Matter and Radiation (PMR-LARN), Namur Research Institute For Life Science (NARILIS), University of Namur, B-5000 Namur, Belgium.
Nanotechnology. 2016 Nov 11;27(45):455101. doi: 10.1088/0957-4484/27/45/455101. Epub 2016 Oct 3.
The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm protons, but not with 10 keV μm protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.
开发新的方法和方案对于改善癌症治疗效果至关重要。鉴于质子具有吸引人的物理特性，以及使用金纳米颗粒（GNPs）具有生物学相关性的新证据，研究人员已经在体外结合不同线性转移能量（LET）的质子束研究了 GNPs（5 或 10nm）的放射增敏作用。在与 GNPs 孵育 24 小时后，暴露于 10nmGNPs 的 A431 细胞的细胞质中以及暴露于 5nmGNPs 的细胞的细胞质和细胞核中观察到了纳米颗粒。细胞摄取 0.05mg/ml 的 GNPs 后，孵育 24 小时后，分别有 0.78pgAu/细胞和 0.30pgAu/细胞。用 25keVμm 质子照射时，GNPs 表现出明显的放射增敏作用，但用 10keVμm 质子照射时则没有。对于 10nmGNPs 比 5nmGNPs 来说，这种作用更为明显。通过使用自由基清除剂，确定了活性氧在辐照细胞死亡放大中的主要作用。总的来说，这些结果为在质子治疗中使用高 Z 金属 NPs 开辟了新的前景。
