Chen Huibin, Wang Meiying, Lin Xiangzhi, Shi Caihua, Liu Zhiyu
Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, 361005, PR China; School of Life Sciences, Xiamen University, Xiamen, 361102, PR China; Biology Department, Xiamen Ocean Vocational College, Xiamen, 361012, PR China.
Biology Department, Xiamen Ocean Vocational College, Xiamen, 361012, PR China.
Food Microbiol. 2017 Feb;61:58-65. doi: 10.1016/j.fm.2016.08.006. Epub 2016 Aug 25.
As filter-feeding bivalves, oysters can accumulate microorganisms into their gills, causing spoilage and potential safety issues. This study aims to investigate the changes in the gill microbiota of oysters packed under air and modified atmospheres (MAs, 50% CO: 50% N, 70% CO: 30% O, and 50% CO: 50% O) during storage at 4 °C. The diversity of bacterial microbiota in oyster gills was profiled through polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis on the 16S rRNA gene V3 region to describe the variation during the entire storage period. The DGGE profile revealed high bacterial diversity in the air- and MA-packaged oyster gills, and the spoilage bacterial microbiota varied in the MA-packaged oyster gills. Results indicated that CO:O (70%:30%) was suitable for oyster MA packaging and that high bacterial loads in oyster gills need to be considered during storage. In addition, Lactobacillus and Lactococcus species were found to grow dominantly in fresh oyster gills under MA packaging, which supports the potential application of MA packaging for oyster storage.
作为滤食性双壳贝类，牡蛎可在其鳃中积累微生物，导致腐败并引发潜在的安全问题。本研究旨在调查在4℃储存期间，置于空气和改良气氛（MA，50% CO: 50% N、70% CO: 30% O和50% CO: 50% O）包装下的牡蛎鳃微生物群的变化。通过对16S rRNA基因V3区域进行聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析，对牡蛎鳃中细菌微生物群的多样性进行了剖析，以描述整个储存期间的变化情况。DGGE图谱显示，空气包装和MA包装的牡蛎鳃中细菌多样性较高，且MA包装的牡蛎鳃中腐败细菌微生物群有所不同。结果表明，CO:O（70%:30%）适用于牡蛎的MA包装，且在储存期间需要考虑牡蛎鳃中较高的细菌载量。此外，发现乳酸杆菌属和乳球菌属在MA包装下的新鲜牡蛎鳃中占主导生长，这支持了MA包装在牡蛎储存中的潜在应用。
