From Medical Affairs (Papa, Blanco), Società Industria Farmaceutica Italiana SpA, and the Ophthalmology Unit (Santocono), Hospital Di Stefano, Catania, Italy.
From Medical Affairs (Papa, Blanco), Società Industria Farmaceutica Italiana SpA, and the Ophthalmology Unit (Santocono), Hospital Di Stefano, Catania, Italy.
J Cataract Refract Surg. 2016 Sep;42(9):1312-1317. doi: 10.1016/j.jcrs.2016.07.022.
To characterize the ocular flora in a consecutive group of patients having cataract surgery and to determine the antibiotic susceptibility profile of isolates to several ophthalmic antibiotics.
Hospital Di Stefano, Catania, Italy.
Observational case series.
Conjunctival and eyelid cultures from patients were obtained 14 days before surgery and, if positive, repeated the day of the surgery. Antimicrobial susceptibility for aminoglycosides (netilmicin and tobramycin), fluoroquinolones (ofloxacin, levofloxacin, and moxifloxacin), chloramphenicol, and azithromycin was tested using the Kirby-Bauer disk diffusion method. Susceptibility was also tested for oxacillin, cefuroxime, and vancomycin. All positive patients received a 2-day preoperative course of 3 mg/mL netilmicin ophthalmic solution 4 times a day. The recovery rate of microorganisms after antibiotic treatment compared with baseline was calculated.
One hundred twenty consecutive patients were included in the study. Cultures were positive in 72.5% of patients; 131 isolates, mainly gram-positive, were identified. Staphylococcus epidermidis (58.0%) and Staphylococcus aureus (15.3%) were the most frequently isolated microorganisms. Methicillin-resistant staphylococci accounted for 3.8% of S epidermidis and 20.0% of S aureus. A high in vitro susceptibility (>90%) for all isolates, including multiresistant coagulase-negative Staphylococcus, was obtained for netilmicin, vancomycin, and cefuroxime. The recovery rate of isolates before surgery was reduced by 93.9% (P < .001).
Conjunctival and lid margin isolates were sensitive to netilmicin, vancomycin, and cefuroxime. Microorganisms were less susceptible to other ophthalmic antibiotics, with the exception of moxifloxacin. A 2-day preoperative course with topical netilmicin reduced most bacteria identified on the conjunctiva and eyelids.
Dr. Papa and Ms. Blanco are employees of Società Industria Farmaceutica Italiana SpA. Dr. Santocono has no financial or proprietary interest in any material or method mentioned.
描述连续接受白内障手术的患者眼部菌群的特征，并确定分离株对几种眼科抗生素的药敏谱。
意大利卡塔尼亚迪斯特凡诺医院。
观察性病例系列。
在术前 14 天从患者的结膜和眼睑获得培养物，如果阳性，则在手术当天重复。使用 Kirby-Bauer 圆盘扩散法测试氨基糖苷类（奈替米星和妥布霉素）、氟喹诺酮类（氧氟沙星、左氧氟沙星和莫西沙星）、氯霉素和阿奇霉素的抗生素敏感性。还测试了耐甲氧西林葡萄球菌对苯唑西林、头孢呋辛和万古霉素的敏感性。所有阳性患者均接受术前 2 天 3mg/mL 奈替米星滴眼液 4 次/天的疗程。计算抗生素治疗后与基线相比微生物的恢复率。
共纳入 120 例连续患者。72.5%的患者培养阳性，共分离出 131 株主要为革兰阳性菌。表皮葡萄球菌（58.0%）和金黄色葡萄球菌（15.3%）是最常分离的微生物。耐甲氧西林葡萄球菌占表皮葡萄球菌的 3.8%和金黄色葡萄球菌的 20.0%。所有分离株（包括多药耐药凝固酶阴性葡萄球菌）对奈替米星、万古霉素和头孢呋辛的体外敏感性均>90%。手术前分离株的恢复率降低了 93.9%（P<0.001）。
结膜和睑缘分离株对奈替米星、万古霉素和头孢呋辛敏感。除莫西沙星外，其他眼科抗生素的敏感性较低。术前 2 天用局部奈替米星治疗可减少结膜和眼睑上大多数细菌。
Papa 博士和 Blanco 女士是 Società Industria Farmaceutica Italiana SpA 的员工。Santocono 博士在本文提到的任何材料或方法中均没有财务或所有权利益。
