Pardridge W M, Yang J, Buciak J, Tourtellotte W W
Department of Medicine, UCLA School of Medicine 90024-1682.
J Neurosci Res. 1989 Jul;23(3):337-41. doi: 10.1002/jnr.490230314.
Antigen presentation within the human central nervous system by the class II histocompatibility DR-antigen may take place at either the brain capillary-endothelial interface or at perivascular cells, such as smooth muscle or pericytes. The present studies employ a new sensitive immunoperoxidase technique, a mouse monoclonal antibody to the human DR-antigen, and microvessels isolated from autopsied human brain. The experiments show that the DR-antigen is readily detectable in human microvasculature of normal brain and is found in the smooth muscle pre-capillary arterioles and capillary pericytes with minimal, if any, staining of capillary endothelium. These results are consistent with the hypothesis that antigen presentation in the CNS occurs primarily at a site immediately distal to the blood--endothelial interface.
Ⅱ类组织相容性DR抗原在人类中枢神经系统内的抗原呈递可能发生在脑毛细血管-内皮界面或血管周围细胞，如平滑肌或周细胞处。本研究采用了一种新的灵敏免疫过氧化物酶技术、一种针对人类DR抗原的小鼠单克隆抗体以及从尸检人脑分离出的微血管。实验表明，DR抗原在正常脑的人类微血管中易于检测到，并且在平滑肌毛细血管前小动脉和毛细血管周细胞中发现，而毛细血管内皮的染色极少（如果有染色的话）。这些结果与中枢神经系统中的抗原呈递主要发生在紧邻血液-内皮界面远端的部位这一假设相一致。
