Boyle E A, McGeer P L
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver.
Am J Pathol. 1990 Sep;137(3):575-84.
Multiple sclerosis plaques were immunohistochemically stained to exhibit cells expressing immune-system antigens. Human leukocyte antigen (HLA)-DR-positive cells formed dense rings around all plaque regions. The majority were reactive microglia/macrophages. Counterstaining with oil red O revealed heavy myelin debris within these cells. They were distinct from astrocytes, which were identified with an antibody to glial fibrillary acidic protein (GFAP) and which did not contain oil red O myelin debris. Numerous leukocytes and microglia were stained with antibody to leukocyte common antigen (LCA). Lymphocytes in cuffs around vessels, along the margins of capillary walls, and, sparingly, in the tissue matrix of affected areas, were stained with antibodies to CD4 (T-helper/inducer) and CD8 (T-cytotoxic/suppressor). In experimental allergic encephalomyelitis (EAE) induced in Lewis rats, a similar proliferation of Ia-positive (OX6, OX17) cells displaying reactive microglia/macrophage morphology was observed. These Ia-positive cells also were easily distinguished from GFAP-positive astrocytes. The results suggest that macrophages/reactive microglia, and not astrocytes, express class II MHC antigens in multiple sclerosis and EAE plaques.
对多发性硬化斑块进行免疫组织化学染色，以显示表达免疫系统抗原的细胞。人类白细胞抗原（HLA）-DR阳性细胞在所有斑块区域周围形成密集环。大多数是反应性小胶质细胞/巨噬细胞。用油红O复染显示这些细胞内有大量髓鞘碎片。它们与星形胶质细胞不同，星形胶质细胞用胶质纤维酸性蛋白（GFAP）抗体鉴定，且不含油红O髓鞘碎片。许多白细胞和小胶质细胞用白细胞共同抗原（LCA）抗体染色。血管周围袖套中的淋巴细胞、沿毛细血管壁边缘以及在受累区域的组织基质中少量淋巴细胞，用CD4（辅助性/诱导性T细胞）和CD8（细胞毒性/抑制性T细胞）抗体染色。在Lewis大鼠诱导的实验性自身免疫性脑脊髓炎（EAE）中，观察到呈现反应性小胶质细胞/巨噬细胞形态的Ia阳性（OX6、OX17）细胞有类似增殖。这些Ia阳性细胞也很容易与GFAP阳性星形胶质细胞区分开来。结果表明，在多发性硬化和EAE斑块中，巨噬细胞/反应性小胶质细胞而非星形胶质细胞表达II类主要组织相容性复合体抗原。
