Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Costal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Sci Rep. 2016 Oct 4;6:34588. doi: 10.1038/srep34588.
Crude oil is a complex mixture of hydrocarbons with different structures; its components vary in bioavailability and toxicity. It is important to understand how bacterial communities response to different hydrocarbons and their co-acclimation in the process of degradation. In this study, microcosms with the addition of structurally different hydrocarbons were setup to investigate the successions of bacterial communities and the interactions between different bacterial taxa. Hydrocarbons were effectively degraded in all microcosms after 40 days. High-throughput sequencing offered a great quantity of data for analyzing successions of bacterial communities. The results indicated that the bacterial communities responded dramatically different to various hydrocarbons. KEGG database and PICRUSt were applied to predict functions of individual bacterial taxa and networks were constructed to analyze co-acclimations between functional bacterial groups. Almost all functional genes catalyzing degradation of different hydrocarbons were predicted in bacterial communities. Most of bacterial taxa were believed to conduct biodegradation processes via interactions with each other. This study addressed a few investigated area of bacterial community responses to structurally different organic pollutants and their co-acclimation and interactions in the process of biodegradation. The study could provide useful information to guide the bioremediation of crude oil pollution.
原油是一种具有不同结构的碳氢化合物的复杂混合物，其成分在生物利用度和毒性方面存在差异。了解细菌群落如何对不同的碳氢化合物做出反应，以及它们在降解过程中的共同适应是很重要的。在这项研究中，通过添加结构不同的碳氢化合物来设置微宇宙，以研究细菌群落的演替以及不同细菌类群之间的相互作用。在 40 天后，所有微宇宙中的碳氢化合物都得到了有效降解。高通量测序为分析细菌群落的演替提供了大量的数据。结果表明，细菌群落对各种碳氢化合物的反应截然不同。KEGG 数据库和 PICRUSt 被用于预测单个细菌类群的功能，并且构建了网络来分析功能细菌群体之间的共同适应。在细菌群落中预测到了几乎所有催化不同碳氢化合物降解的功能基因。大多数细菌类群被认为通过相互作用进行生物降解过程。本研究探讨了细菌群落对结构不同的有机污染物的反应以及它们在生物降解过程中的共同适应和相互作用的一些研究领域。该研究可以为指导原油污染的生物修复提供有用的信息。
