Deitch A D, Strand M A, de Vere White R W
Department of Urology, University of California School of Medicine, Davis.
J Urol. 1989 Sep;142(3):759-62. doi: 10.1016/s0022-5347(17)38879-1.
Deoxyribonucleic acid flow cytometry was performed on aspirated prostatic cells from 198 patients who had benign cytological or histological findings. Unsatisfactory acellular histograms were obtained from 10.6 per cent of the cases. Three-fourths of the satisfactory samples (more than 5,000 cells after subtracting debris) showed the expected single peak deoxyribonucleic acid diploid to near diploid histograms. Unexpectedly, the remaining samples were deoxyribonucleic acid aneuploid, most having 2 peridiploid peaks (deoxyribonucleic acid index 0.82 to 1.31). Usually, proliferation was low with less than 20 per cent hyperdiploid cells and with 2.5 +/- 1.5 per cent G2 cells. In 10 per cent of the single peak histograms there was evidence of inflammation, identified as an increase in hyperdiploid cells without an increased percentage of G2 cells but with a tail of high channel values. The aforementioned histogram features were considered to be benign findings. Seven per cent of the samples had deoxyribonucleic acid histograms suggestive of prostate cancer. Of these samples 7 had diploid or peridiploid aneuploid histograms with high proliferation (more than 20 per cent hyperdiploid cells with 8.5 +/- 3.8 per cent G2 cells), while 5 had histograms with deoxyribonucleic acid aneuploidy other than peridiploidy.
对198例细胞学或组织学检查结果为良性的患者的前列腺穿刺细胞进行了脱氧核糖核酸流式细胞术检测。10.6%的病例获得的无细胞直方图不理想。四分之三的满意样本（减去碎片后超过5000个细胞）呈现出预期的单峰脱氧核糖核酸二倍体至近二倍体直方图。出乎意料的是，其余样本为脱氧核糖核酸非整倍体，大多数有2个亚二倍体峰（脱氧核糖核酸指数0.82至1.31）。通常，增殖率较低，超二倍体细胞少于20%，G2期细胞为2.5±1.5%。在10%的单峰直方图中，有炎症迹象，表现为超二倍体细胞增加，G2期细胞百分比未增加，但有高通道值的拖尾。上述直方图特征被认为是良性表现。7%的样本的脱氧核糖核酸直方图提示前列腺癌。在这些样本中，7个具有二倍体或亚二倍体非整倍体直方图且增殖率高（超二倍体细胞超过20%，G2期细胞为8.5±3.8%），而5个具有非亚二倍体的脱氧核糖核酸非整倍体直方图。
