Müller J G, Demel S, Wirth M P, Manseck A, Frohmüller H G, Müller H A
Institute of Pathology, University of Würzburg, Germany.
Virchows Arch. 1994;424(6):647-51. doi: 10.1007/BF00195780.
Paraffin embedded tissue of 49 stage C and 27 stage B prostate adenocarcinomas was investigated by flow cytometry. All patients were treated by radical prostatectomy with pelvic lymphadenectomy and followed up for 5-10 years. The tumour was separated from the benign tissue to increase the proportion of tumour cells. Ten stage C and seven stage B carcinomas had to be excluded because of poor fixation. Six of the 39 (15%) stage C and 1/20 (5%) stage B carcinomas were aneuploid. Cell cycle analysis was done with correction for sliced nuclei and background subtraction. The threshold between carcinomas with low and with increased ("tetraploid") G2M-fraction was determined by comparing carcinomas with and without tumour progression. Sixty-seven percent of the patients with non-euploid stage C carcinomas and 11% of those with euploid carcinomas suffered from tumour progression (P < 0.01). The respective values for the stage B carcinomas were 67% and 6% (P < 0.01). These results demonstrate the strong prognostic impact of DNA-ploidy and G2M-fractions for each individual patient.
通过流式细胞术对49例C期和27例B期前列腺腺癌的石蜡包埋组织进行了研究。所有患者均接受了前列腺癌根治术及盆腔淋巴结清扫术，并随访5至10年。将肿瘤与良性组织分离以增加肿瘤细胞的比例。由于固定不佳，10例C期和7例B期癌不得不被排除。39例C期癌中的6例（15%）和20例B期癌中的1例（5%）为非整倍体。细胞周期分析采用切片核校正和背景扣除。通过比较有和无肿瘤进展的癌来确定低G2M分数和增加的（“四倍体”）G2M分数的癌之间的阈值。非整倍体C期癌患者中有67%和整倍体癌患者中有11%出现肿瘤进展（P<0.01）。B期癌的相应数值分别为67%和6%（P<0.01）。这些结果证明了DNA倍性和G2M分数对每个患者的强烈预后影响。
