Zhou Yang, Liu Zonglin, Chen Yuchen, Jin Li Hua
College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China.
Exp Ther Med. 2016 Oct;12(4):2671-2680. doi: 10.3892/etm.2016.3641. Epub 2016 Aug 31.
Traditional medicinal plants are widely used as immunomodulatory medicines that help improve health. A total of 50 different plants used for the treatment of toxicity were screened for their protective effects. Flies were fed a standard cornmeal-yeast medium (control group) or the standard medium containing medicinal plant extracts (experimental groups). Assessment of the survival rate was performed by feeding flies with toxic compounds. Gut epithelial cells were analyzed for cell proliferation and death by green fluorescent protein antibodies and 7-aminoactinomycin D staining under the microscope. The expression of antimicrobial peptides (AMPs) was evaluated by the quantitative polymerase chain reaction and the results revealed that after feeding the flies with toxic compounds, aqueous extracts from (Franch.) Nannf (), (Decne.) C.B.Clarke (), Beauv.var. (Nees) C.E. Hubb. ( var. ) and Sied. Et Zucc. () increased the fly survival rate, reduced epithelial cell death and improved gut morphology. In addition, extracts induced the antimicrobial peptide levels (Dpt and Mtk) following treatment with sodium dodecyl sulfate (SDS). However, these extracts were not observed to increase SDS-induced cell proliferation . These results indicate that there are strong protective effects in extracts of , , var. and on intestinal cells among 50 medicinal plants.
传统药用植物被广泛用作免疫调节药物，有助于改善健康。对总共50种用于治疗毒性的不同植物进行了保护作用筛选。给果蝇喂食标准玉米粉-酵母培养基（对照组）或含有药用植物提取物的标准培养基（实验组）。通过给果蝇喂食有毒化合物来评估存活率。在显微镜下用绿色荧光蛋白抗体和7-氨基放线菌素D染色分析肠道上皮细胞的细胞增殖和死亡情况。通过定量聚合酶链反应评估抗菌肽（AMPs）的表达，结果显示，在用有毒化合物喂食果蝇后，鸡屎藤（Paederia scandens (Lour.) Merr.）、头花蓼（Polygonum capitatum Buch.-Ham. ex D. Don）、少花龙葵（Solanum nigrum L. var. pauciflorum Liou）和毛曼陀罗（Datura innoxia Mill.）的水提取物提高了果蝇的存活率，减少了上皮细胞死亡并改善了肠道形态。此外，毛曼陀罗提取物在用十二烷基硫酸钠（SDS）处理后诱导了抗菌肽水平（Dpt和Mtk）。然而，未观察到这些提取物增加SDS诱导的细胞增殖。这些结果表明，在50种药用植物中，鸡屎藤、头花蓼、少花龙葵和毛曼陀罗的提取物对果蝇肠道细胞有很强的保护作用。
