Boccara Martine, Fedala Yasmina, Bryan Catherine Venien, Bailly-Bechet Marc, Bowler Chris, Boccara Albert Claude
Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR 8197, INSERM U1024, 46 rue d'Ulm, F-75005 Paris, France; Atelier de Bioinformatique, UMR 7205 ISYEB CNRS, MNHN, UPMC, EPHE, 45 rue Buffon, 75005 Paris, France; These authors contributed equally to this paper;
Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR 8197, INSERM U1024, 46 rue d'Ulm, F-75005 Paris, France; Institut Langevin, ESPCI ParisTech, PSL Research University, CNRS UMR 7587, 1 rue Jussieu, 75005 Paris, France; These authors contributed equally to this paper.
Biomed Opt Express. 2016 Aug 29;7(9):3736-3746. doi: 10.1364/BOE.7.003736. eCollection 2016 Sep 1.
There is a huge abundance of viruses and membrane vesicles in seawater. We describe a new full-field, incoherently illuminated, shot-noise limited, common-path interferometric detection method that we couple with the analysis of Brownian motion to detect, quantify, and differentiate biotic nanoparticles. We validated the method with calibrated nanoparticles and homogeneous DNA or RNA viruses. The smallest virus size that we characterized with a suitable signal-to-noise ratio was around 30 nm in diameter. Analysis of Brownian motions revealed anisotropic trajectories for myoviruses.We further applied the method for vesicles detection and for analysis of coastal and oligotrophic samples from Oceans circumnavigation.
海水中存在大量的病毒和膜泡。我们描述了一种新的全场、非相干照明、散粒噪声受限的共光路干涉检测方法，该方法与布朗运动分析相结合，用于检测、量化和区分生物纳米颗粒。我们用校准的纳米颗粒以及均质DNA或RNA病毒对该方法进行了验证。我们以合适的信噪比表征的最小病毒大小约为直径30纳米。对布朗运动的分析揭示了肌尾噬菌体的各向异性轨迹。我们进一步将该方法应用于膜泡检测以及对环球海洋的沿海和贫营养样品的分析。
