Hsu B Y, Lin S W, Inbaraj B Stephen, Chen B H
Department of Food and Beverage Management, Lee-Ming Institute of Technology, New Taipei City 242, Taiwan.
Department of Food Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.
J Pharm Biomed Anal. 2017 Jan 5;132:109-116. doi: 10.1016/j.jpba.2016.09.027. Epub 2016 Sep 26.
A high performance liquid chromatography-diode array detection-tandem mass spectrometry method (HPLC-DAD-MS/MS) was developed for simultaneous determination of phenolic acids and flavonoids in djulis (Chenopodium formosanum Koidz.), a traditional Chinese herb reported to possess vital biological activities. A high yield of phenolic acids and flavonoids was attained by employing 50% ethanol in water as the extraction solvent and shaking in a 60°C water bath for 3h. A total of 8 phenolic acids and 14 flavonoids were separated and identified within 55min by using a Poroshell 120 EC-C18 column with detection at 280nm, flow rate at 0.8mL/min, column temperature at 35°C, and a gradient solvent system of 0.1% formic acid in water and acetonitrile. Two internal standards caffeic acid and kaempferol-3-O-rutinoside were used for quantitation of phenolic acids and flavonoids in djulis respectively. The amounts of phenolic acids ranged from 11.5±0.8μg/g (caffeoyl-putrescine-derivative (2)) to 1855.3±16.9μg/g (hydroxylphenylacetic acid pentoside), while the flavonoids ranged from 19.93±2.29μg/g (quercetin-3-O-(coumaryl)-rutinoside-pentoside (1)) to 257.3±2.05μg/g (rutin-O-pentoside (2)). A high recovery (89.68-97.20%) and high reproducibility was obtained for both phenolic acids and flavonoids with the relative standard deviation (RSD) for the latter ranging from 0.09-8.22% (intra-day variability) and 0.80-8.48% (inter-day variability). This method may be applied to determination of both phenolic acids and flavonoids in food products and Chinese herbs.
建立了一种高效液相色谱 - 二极管阵列检测 - 串联质谱法（HPLC - DAD - MS/MS），用于同时测定藜麦（Chenopodium formosanum Koidz.）中的酚酸和黄酮类化合物，藜麦是一种据报道具有重要生物活性的传统中草药。以50%乙醇水溶液为提取溶剂，在60°C水浴中振荡提取3小时，可获得较高产量的酚酸和黄酮类化合物。使用Poroshell 120 EC - C18色谱柱，在280nm波长下检测，流速为0.8mL/min，柱温为35°C，以0.1%甲酸水溶液和乙腈为梯度洗脱溶剂系统，在55分钟内分离并鉴定出8种酚酸和14种黄酮类化合物。分别使用两种内标物咖啡酸和山柰酚 - 3 - O - 芸香糖苷对藜麦中的酚酸和黄酮类化合物进行定量。酚酸含量范围为11.5±0.8μg/g（咖啡酰腐胺衍生物（2））至1855.3±16.9μg/g（羟基苯乙酸戊糖苷），而黄酮类化合物含量范围为19.93±2.29μg/g（槲皮素 - 3 - O -（香豆酰） - 芸香糖苷 - 戊糖苷（1））至257.3±2.05μg/g（芦丁 - O - 戊糖苷（2））。酚酸和黄酮类化合物均具有较高的回收率（89.68 - 97.20%）和重现性，黄酮类化合物的相对标准偏差（RSD）在日内变化范围为0.09 - 8.22%，日间变化范围为0.80 - 8.48%。该方法可应用于食品和中草药中酚酸和黄酮类化合物的测定。
