Sabahi Zahra, Farmani Fatemeh, Soltani Fatemeh, Moein Mahmoodreza
Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Fars, Iran.
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2018 Apr;21(4):411-416. doi: 10.22038/IJBMS.2018.26563.6506.
The aim of this study was to prepare fraction and determine the biological activities of the polyphenol-enriched fraction of Bunge fruits.
In this assay fraction was extracted by column chromatography, using Amberlite column as the stationary phase. Phenol and flavonoids in the extract and fraction were analyzed by high performance liquid chromatography (HPLC). DNA protection ability, antioxidant and xanthine oxidase inhibition capacities of this fraction were also examined.
Phenol and flavonoid content measurement and HPLC analyses of this fraction confirmed that phenol and flavonoids were increased in fraction in comparison to extract (before using Amberlite column). In antioxidant measurement assay, the trolox equivalent values were 1.05± 0.04 and 0.8±0.11 in oxygen radical absorbance capacity (ORAC) and the EC values for cellular antioxidant activity were 55.51±0.21 and 95.67±0.13 µg/ml for quercetin and the fraction, respectively. The xanthine oxidase inhibition percentages were 97.6±0.003 and 90.2 6±0.003 in 100 µg/ml concentration of fraction and vitamin C respectively. Comet assay analysis showed that this fraction protects human lymphocytes against HO-induced DNA damages at 12.5 to 100 µg/ml concentrations.
This study suggests that Amberlite column as the stationary phase help to improve phenolic compound in separating fractions. The results showed that fruits are rich in phenolic compounds and they are potent antioxidants with protective effects on oxidative damages. They might be used as functional ingredients in food and supplements.
本研究旨在制备 Bunge 果实富含多酚部分并测定其生物活性。
在本实验中，使用 Amberlite 柱作为固定相，通过柱色谱法提取部分。采用高效液相色谱（HPLC）分析提取物和部分中的酚类和黄酮类化合物。还检测了该部分的 DNA 保护能力、抗氧化能力和黄嘌呤氧化酶抑制能力。
该部分的酚类和黄酮类含量测定及 HPLC 分析证实，与提取物（使用 Amberlite 柱之前）相比，部分中的酚类和黄酮类含量有所增加。在抗氧化测定中，氧自由基吸收能力（ORAC）的 Trolox 当量值分别为 1.05±0.04 和 0.8±0.11，槲皮素和该部分的细胞抗氧化活性的 EC 值分别为 55.51±0.21 和 95.67±0.13 μg/ml。在 100 μg/ml 浓度下，该部分和维生素 C 的黄嘌呤氧化酶抑制率分别为 97.6±0.003 和 90.26±0.003。彗星试验分析表明，该部分在 12.5 至 100 μg/ml 浓度下可保护人类淋巴细胞免受 HO 诱导的 DNA 损伤。
本研究表明，以 Amberlite 柱作为固定相有助于在分离部分中提高酚类化合物含量。结果表明，Bunge 果实富含酚类化合物，它们是具有抗氧化作用的强效抗氧化剂，对氧化损伤具有保护作用。它们可作为食品和补充剂中的功能成分。
