Department of Gastroenterology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, Zhejiang province 310016, China.
Division of Gastroenterology, Loma Linda University, Loma Linda, CA 92354, USA.
Sci Rep. 2016 Oct 6;6:34305. doi: 10.1038/srep34305.
Functional dyspepsia (FD) is a functional gastrointestinal disorder diagnosed by symptom-based criteria. It has been said that duodenal immune activation plays a role in the pathogenesis of FD. The primary aims of the study were to compare the total number of duodenal eosinophil and evaluate the eosinophil degranulation rate, number of duodenal degranulated eosinophil and mast cell between patients with FD and healthy subjects. We enrolled 96 patients with FD and 24 healthy controls at Sir Run Run Shaw Hospital. The total number of eosinophil was comparable in the second portion of duodenum (D2) and duodenal bulb (D1) between patients with FD and healthy controls (all P > 0.05). Significant higher eosinophil degranulation positive rate in D2 (P = 0.003) and a trend towards higher in D1 (P = 0.084) were observed in patients with FD compared with healthy controls. Moreover, the number of duodenal degranulated eosinophil in patients with FD were significantly increased than healthy controls in D1(9.8 ± 6.3 vs 2.9 ± 2.1 per HPF, P = 0.0002) and a trend towards increase in D2 (10.7 ± 7.7 vs 5.3 ± 0.9 per HPF, P = 0.077), respectively. However, degranulated mast cells in patients with FD were almost same with healthy controls. Increased eosinophils degranulation in duodenum play an important role in pathogenesis of FD.
功能性消化不良（FD）是一种基于症状标准诊断的功能性胃肠道疾病。有人说十二指肠免疫激活在 FD 的发病机制中起作用。该研究的主要目的是比较十二指肠嗜酸性粒细胞的总数，并评估 FD 患者与健康受试者之间的嗜酸性粒细胞脱颗粒率、脱颗粒嗜酸性粒细胞和肥大细胞的数量。我们在浙江大学医学院附属邵逸夫医院招募了 96 名 FD 患者和 24 名健康对照者。FD 患者和健康对照组的十二指肠第二段（D2）和十二指肠球部（D1）的嗜酸性粒细胞总数无差异（均 P>0.05）。与健康对照组相比，FD 患者的 D2 嗜酸性粒细胞脱颗粒阳性率显著升高（P=0.003），D1 呈升高趋势（P=0.084）。此外，FD 患者 D1 的脱颗粒嗜酸性粒细胞数量明显高于健康对照组（9.8±6.3 比 2.9±2.1 个/高倍视野，P=0.0002），D2 也呈升高趋势（10.7±7.7 比 5.3±0.9 个/高倍视野，P=0.077）。然而，FD 患者的脱颗粒肥大细胞与健康对照组几乎相同。十二指肠嗜酸性粒细胞脱颗粒增加在 FD 的发病机制中起重要作用。
