College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center For Regional Environmental Quality, Tongji University, Shanghai 200092, China.
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center For Regional Environmental Quality, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2017 Sep;239:394-401. doi: 10.1016/j.biortech.2017.04.070. Epub 2017 Apr 21.
The lower utilization ratios of high-content VFAs resulted in less lipid yield by the oleaginous yeast Cryptococcus curvatus ATCC 20509. In this work, increasing the inoculation concentration to OD600=5.1 improved the acetic acid utilization ratio (99.8%) and lipids production (4.63g/L) in mediums with 30g/L of acetic acid. For the higher acetic acid concentration (40g/L), increasing the nitrogen to carbon ratio (0.033) and raising the initial pH (pH=8) was superior over improvement in the inoculate, with the lipid production increased from 1.08g/L to 6.49g/L. Subsequently, mixed VFAs at concentrations of 30g/L and 40g/L were used as the carbon source to simulate waste-derived VFAs. High lipid production (4.82 and 7.45g/L, respectively) was correspondingly achieved with similar high lipid yield (0.187g/g). This study provides an effective strategy to enhance the bioconversion of high-content VFAs into microbial lipids.
高含量 VFAs 的低利用率导致产油酵母Cryptococcus curvatus ATCC 20509 的油脂产量较低。在这项工作中，将接种浓度提高到 OD600=5.1，可提高 30g/L 乙酸介质中的乙酸利用率（99.8%）和油脂产量（4.63g/L）。对于更高的乙酸浓度（40g/L），提高氮碳比（0.033）和初始 pH（pH=8）优于提高接种量，油脂产量从 1.08g/L 增加到 6.49g/L。随后，将浓度为 30g/L 和 40g/L 的混合 VFAs 用作碳源来模拟废衍生 VFAs。高含量 VFAs（分别为 30g/L 和 40g/L）的高油脂产量（分别为 4.82 和 7.45g/L），相应地也实现了类似的高油脂产率（0.187g/g）。本研究提供了一种有效策略，可增强高含量 VFAs 向微生物油脂的生物转化。
