Vinceković Marko, Jalšenjak Nenad, Topolovec-Pintarić Snježana, Đermić Edyta, Bujan Marija, Jurić Slaven
Department of Chemistry and ‡Department of Plant Pathology, University of Zagreb Faculty of Agriculture , 10000 Zagreb, Croatia.
J Agric Food Chem. 2016 Nov 2;64(43):8073-8083. doi: 10.1021/acs.jafc.6b02879. Epub 2016 Oct 19.
Novel chitosan/alginate microcapsules simultaneously loaded with copper cations and Trichoderma viride have been prepared and characterized. Information about the intermolecular interactions between biopolymers and bioactive agents was obtained by Fourier transform infrared spectroscopy. Encapsulation of T. viride spores and the presence of copper cations in the same compartment does not inhibit their activity. Microcapsule loading capacity and efficiency as well as swelling behavior and release depend on both the size of the microcapsule and bioactive agents. The in vitro copper cation release profile was fitted to a Korsmeyer-Peppas empirical model. Fickian diffusion was found to be a rate-controlling mechanism of release from smaller microcapsules, whereas anomalous transport kinetics controlled release from larger microcapsules. The T. viride spore release profile exhibited exponential release over the initial lag time. The results obtained opened perspectives for the future use of chitosan/alginate microcapsules simultaneously loaded with biological and chemical agents in plant nutrition and protection.
已制备并表征了同时负载铜离子和绿色木霉的新型壳聚糖/海藻酸盐微胶囊。通过傅里叶变换红外光谱获得了生物聚合物与生物活性剂之间分子间相互作用的信息。绿色木霉孢子的包封以及同一隔室中铜离子的存在并不抑制它们的活性。微胶囊的负载能力和效率以及溶胀行为和释放取决于微胶囊和生物活性剂的大小。体外铜离子释放曲线符合Korsmeyer-Peppas经验模型。发现菲克扩散是较小微胶囊释放的速率控制机制，而反常传输动力学控制较大微胶囊的释放。绿色木霉孢子释放曲线在初始滞后时间内呈现指数释放。所获得的结果为未来在植物营养和保护中同时负载生物和化学试剂的壳聚糖/海藻酸盐微胶囊的应用开辟了前景。
