2015年泰国、越南和老挝人民民主共和国猪三角洲冠状病毒的不同谱系
Different Lineage of Porcine Deltacoronavirus in Thailand, Vietnam and Lao PDR in 2015.
作者信息
Saeng-Chuto K, Lorsirigool A, Temeeyasen G, Vui D T, Stott C J, Madapong A, Tripipat T, Wegner M, Intrakamhaeng M, Chongcharoen W, Tantituvanont A, Kaewprommal P, Piriyapongsa J, Nilubol D
机构信息
Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Virology Section, Department of Animal Health, National Center for Veterinary Diagnosis, Hanoi, Vietnam.
出版信息
Transbound Emerg Dis. 2017 Feb;64(1):3-10. doi: 10.1111/tbed.12585. Epub 2016 Oct 7.
Abstract
Porcine deltacoronavirus (PDCoV) was detected by RT-PCR in 12 of 97 (12.4%) intestinal samples collected during 2015 from piglets with diarrhoea in Thailand, Vietnam and Lao PDR. Spike, membrane and nucleocapsid genes were characterized, and phylogenetic analyses demonstrated that PDCoV isolates from Thai and Lao PDR form a novel cluster, separated from US and China isolates, but relatively were more closely related to China PDCoV than US isolates. Vietnam PDCoVs, however, were grouped together with US PDCoV. The analyses of amino acid changes suggested that they were from different lineage.
摘要
2015年期间，在泰国、越南和老挝人民民主共和国采集的97份腹泻仔猪肠道样本中，有12份（12.4%）通过逆转录聚合酶链反应（RT-PCR）检测到猪德尔塔冠状病毒（PDCoV）。对刺突蛋白、膜蛋白和核衣壳蛋白基因进行了特征分析，系统发育分析表明，来自泰国和老挝人民民主共和国的PDCoV分离株形成一个新的聚类，与美国和中国的分离株不同，但与中国的PDCoV分离株相比，与美国的分离株亲缘关系更近。然而，越南的PDCoV与美国的PDCoV归为一组。氨基酸变化分析表明它们来自不同的谱系。
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