Hilly Ohad, Strenov Yulia, Rath-Wolfson Lea, Hod Roy, Shkedy Yotam, Mizrachi Aviram, Koren Rumelia, Shpitzer Thomas
Departments of Otolaryngology, Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Departments of Pathology, Rabin Medical Center, Petach Tikva, Affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Pathol Res Pract. 2016 Dec;212(12):1138-1143. doi: 10.1016/j.prp.2016.09.011. Epub 2016 Sep 23.
The aim of this study was to evaluate the relationship between dendritic cell density in early squamous cell carcinoma (SCC) of the tongue and patients' clinical outcome.
Representative samples of low-risk SCC of the tongue (T1-2,N0,M0) from a homogeneous group of 18 patients following local complete excision and elective selective neck dissection, were immunostained with antibodies against S100 and CD1a. Dendritic cell density was analyzed by outcome.
Mean dendritic cell densities were 17 cells/HPF for tumoral S100 and CD1a counts, and 10 cells/HPF for peritumoral S100 and CD1a counts. Better disease-free survival was associated with low peritumoral S100- and CD1a- positive cell counts (p=0.006 and p=0.004, respectively), and with low tumoral S100- and CD1a- positive cell counts (p=0.037 and p=0.04, respectively). Lymphocytic response was decreased in tumors with high dendritic cell density (p=NS). There was no association of dendritic cell density with patient age, tumor size and depth of invasion.
These results may suggest an association between dendritic cell accumulation and functional immunologic impairment.
本研究旨在评估早期舌鳞状细胞癌（SCC）中树突状细胞密度与患者临床结局之间的关系。
对18例局部完全切除并进行选择性颈部清扫的低风险舌SCC（T1-2，N0，M0）患者的代表性样本，用抗S100和CD1a抗体进行免疫染色。根据结局分析树突状细胞密度。
肿瘤S100和CD1a计数的平均树突状细胞密度为17个细胞/高倍视野（HPF），肿瘤周围S100和CD1a计数为10个细胞/HPF。更好的无病生存率与肿瘤周围低S100和CD1a阳性细胞计数相关（分别为p = 0.006和p = 0.004），以及与肿瘤低S100和CD1a阳性细胞计数相关（分别为p = 0.037和p = 0.04）。在树突状细胞密度高的肿瘤中淋巴细胞反应降低（p =无显著性差异）。树突状细胞密度与患者年龄、肿瘤大小和浸润深度无关。
这些结果可能提示树突状细胞聚集与功能性免疫损伤之间存在关联。
