Department of Chemistry, College of Arts and Sciences, Khalifa University of Science and Technology, Abu Dhabi P.O. Box 127788, United Arab Emirates.
Center for Membranes and Advanced Water Technology (CMAT), Khalifa University of Science and Technology, Abu Dhabi P.O. Box 127788, United Arab Emirates.
Biomolecules. 2021 Apr 29;11(5):656. doi: 10.3390/biom11050656.
In recent years, concerns are being raised about the potential harmful effects of emerging pollutants (EPs) on human and aquatic lives. Extensive research is being conducted on developing efficient remediation strategies to target this new class of toxic pollutants. Studies focused on biological (enzyme-based) methods have shown potential as greener and possibly more economical alternatives to other treatment approaches, such as chemical methods. The current study focused on the use of recombinantly produced novel bacterial peroxidases, namely dye-decolorizing peroxidases (DyPs), to study their effectiveness in degrading a number of diverse EPs. In this context, a sensitive bioanalytical Liquid chromatography-tandem mass spectrometry (LCMSMS)-based method was developed to simultaneously detect a mixture of 31 EPs and to examine their degradability by a panel of seven different recombinant bacterial DyPs (rDyPs). We show that up to 9 of the 31 tested EPs could be degraded by at least one of the DyPs tested. The results also indicated that not all rDyPs behaved similarly in their abilities to degrade EPs, as some rDyPs (such as DyP and DyP) showed a promising potential to degrade EPs while others (such as DyP) were almost ineffective. Additionally, the role of redox mediators for effective emerging pollutant degradation by rDyPs was also examined, which showed dramatic improvement in the DyP-mediated degradation of five different EPs. Detailed analysis of 2-mercaptobenzothiazole degradation by DyP showed that six distinct breakdown products were generated. The present study showed for the first time that recombinant bacterial DyPs can be used for wastewater remediation by degrading a range of different EPs.
近年来，人们对新兴污染物 (EPs) 对人类和水生生物的潜在有害影响表示担忧。目前正在进行广泛的研究，以开发针对这种新型有毒污染物的有效修复策略。研究集中在生物（基于酶的）方法上，这些方法已显示出作为比其他处理方法（如化学方法）更环保且可能更经济的替代方法的潜力。本研究集中于使用重组生产的新型细菌过氧化物酶，即染料脱色过氧化物酶 (DyP)，研究它们在降解多种不同 EPs 方面的有效性。在这种情况下，开发了一种灵敏的基于液相色谱-串联质谱 (LCMSMS) 的生物分析方法，以同时检测 31 种 EPs 的混合物，并检查七种不同重组细菌 DyP (rDyP) 对其降解能力。我们表明，多达 31 种测试的 EPs 中的 9 种可以被至少一种测试的 DyP 降解。结果还表明，并非所有 rDyP 在降解 EPs 的能力方面表现相同，因为一些 rDyP（如 DyP 和 DyP）在降解 EPs 方面表现出很大的潜力，而其他 rDyP（如 DyP）几乎无效。此外，还研究了氧化还原介体在 rDyP 有效降解新兴污染物中的作用，这表明氧化还原介体显著提高了 DyP 对五种不同 EPs 的降解能力。DyP 对 2-巯基苯并噻唑的降解的详细分析表明，生成了六个不同的分解产物。本研究首次表明，重组细菌 DyP 可通过降解一系列不同的 EPs 用于废水修复。
