Kerr G J, McCann M R, Branch J K, Ratneswaran A, Pest M A, Holdsworth D W, Beier F, Dixon S J, Séguin C A
Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada; Bone and Joint Institute, The University of Western Ontario, London, Ontario, Canada.
School of Dentistry, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
Osteoarthritis Cartilage. 2017 Mar;25(3):421-425. doi: 10.1016/j.joca.2016.09.020. Epub 2016 Oct 6.
Whole-body vibration (WBV) platforms are commercially available devices that are used clinically to treat numerous musculoskeletal conditions based on their reported ability to increase bone mineral density and muscle strength. Despite widespread use, there is an alarming lack of understanding of the direct effects of WBV on joint health. Previous work by our lab demonstrated that repeated exposure to WBV using protocols that model those used clinically, induces intervertebral disc (IVD) degeneration and osteoarthritis-like damage in the knee of skeletally mature, male mice of a single outbred strain (CD-1). The present study examined whether exposure to WBV induces similar deleterious effects in a genetically different strain of mouse (C57BL/6).
Male 10-week-old C57BL/6 mice were exposed to vertical sinusoidal WBV for 30 min/day, 5 days/week, for 4 or 8 weeks using previously reported protocols (45 Hz, 0.3 g peak acceleration). Following WBV, joint tissues were examined using histological analysis and gene expression was quantified using real-time PCR (qPCR).
Our analyses show a lack of WBV-induced degeneration in either the knee or IVDs of C57BL/6 mice exposed to WBV for 4 or 8 weeks, in direct contrast to the WBV-induced damage previously reported by our lab in CD-1 mice.
Together with previous studies from our group, the present study demonstrates that the effects of WBV on joint tissues vary in a strain-specific manner. These findings highlight the need to examine genetic or physiological differences that may underlie susceptibility to the deleterious effects of WBV on joint tissues.
全身振动（WBV）平台是市面上可买到的设备，基于其能提高骨矿物质密度和肌肉力量的报道，临床上被用于治疗多种肌肉骨骼疾病。尽管被广泛使用，但人们对WBV对关节健康的直接影响却缺乏令人担忧的了解。我们实验室之前的研究表明，使用模拟临床使用的方案反复暴露于WBV，会在骨骼成熟的单一远交系（CD-1）雄性小鼠的膝关节中诱发椎间盘（IVD）退变和骨关节炎样损伤。本研究检测了暴露于WBV是否会在基因不同的小鼠品系（C57BL/6）中诱发类似的有害影响。
10周龄雄性C57BL/6小鼠按照之前报道的方案（45Hz，0.3g峰值加速度），每天暴露于垂直正弦波WBV 30分钟，每周5天，持续4周或8周。WBV处理后，使用组织学分析检查关节组织，并使用实时PCR（qPCR）对基因表达进行定量。
我们的分析表明，暴露于WBV 4周或8周的C57BL/6小鼠的膝关节或IVD中均未出现WBV诱导的退变，这与我们实验室之前报道的CD-1小鼠中WBV诱导的损伤形成直接对比。
结合我们小组之前的研究，本研究表明WBV对关节组织的影响因品系而异。这些发现凸显了需要研究可能是WBV对关节组织产生有害影响易感性基础的遗传或生理差异。
