University of Western Ontario Schulich School of Medicine and Dentistry, London, Ontario, Canada.
Shriners Hospital for Children and McGill University, Montreal, Quebec, Canada.
Arthritis Rheumatol. 2015 May;67(8):2164-75. doi: 10.1002/art.39154.
High-frequency, low-amplitude whole-body vibration (WBV) is being used to treat a range of musculoskeletal disorders; however, there is surprisingly limited knowledge regarding its effect(s) on joint tissues. This study was undertaken to examine the effects of repeated exposure to WBV on bone and joint tissues in an in vivo mouse model.
Ten-week-old male mice were exposed to vertical sinusoidal vibration under conditions that mimic those used clinically in humans (30 minutes per day, 5 days per week, at 45 Hz with peak acceleration at 0.3g). Following WBV, skeletal tissues were examined by micro-computed tomography, histologic analysis, and immunohistochemistry, and gene expression was quantified using real-time polymerase chain reaction.
Following 4 weeks of WBV, intervertebral discs showed histologic hallmarks of degeneration in the annulus fibrosus, disruption of collagen organization, and increased cell death. Greater Mmp3 expression in the intervertebral disc, accompanied by enhanced collagen and aggrecan degradation, was found in mice exposed to WBV as compared to controls. Examination of the knee joints after 4 weeks of WBV revealed meniscal tears and focal damage to the articular cartilage, changes resembling osteoarthritis. Moreover, mice exposed to WBV also demonstrated greater Mmp13 gene expression and enhanced matrix metalloproteinase-mediated collagen and aggrecan degradation in articular cartilage as compared to controls. No changes in trabecular bone microarchitecture or density were detected in the proximal tibia.
Our experiments reveal significant negative effects of WBV on joint tissues in a mouse model. These findings suggest the need for future studies of the effects of WBV on joint health in humans.
高频低幅全身振动（WBV）被用于治疗多种肌肉骨骼疾病；然而，人们对其对关节组织的影响知之甚少。本研究旨在研究重复暴露于 WBV 对体内小鼠模型中骨骼和关节组织的影响。
10 周龄雄性小鼠在模拟人体临床应用条件下（每天 30 分钟，每周 5 天，频率为 45Hz，峰值加速度为 0.3g）接受垂直正弦振动。在 WBV 之后，通过微计算机断层扫描、组织学分析和免疫组织化学检查来检查骨骼组织，使用实时聚合酶链反应来定量基因表达。
在接受 4 周的 WBV 后，椎间盘的纤维环出现组织学退变特征，胶原组织紊乱，细胞死亡增加。与对照组相比，在接受 WBV 的小鼠中，椎间盘的 Mmp3 表达增加，伴随胶原和聚集蛋白降解增强。在接受 4 周的 WBV 后检查膝关节发现半月板撕裂和关节软骨的局灶性损伤，这些变化类似于骨关节炎。此外，与对照组相比，接受 WBV 的小鼠还表现出关节软骨中 Mmp13 基因表达增加和基质金属蛋白酶介导的胶原和聚集蛋白降解增强。在胫骨近端未检测到骨小梁微观结构或密度的变化。
我们的实验揭示了 WBV 对小鼠模型中关节组织的显著负面影响。这些发现提示需要进一步研究 WBV 对人类关节健康的影响。
