红细胞制品中的细胞外囊泡会引发强烈的促炎宿主反应，这取决于数量和储存时间。
Extracellular Vesicles from Red Blood Cell Products Induce a Strong Pro-Inflammatory Host Response, Dependent on Both Numbers and Storage Duration.
作者信息
Straat Marleen, Böing Anita N, Tuip-De Boer Anita, Nieuwland Rienk, Juffermans Nicole P
机构信息
Department of Intensive Care Medicine, Academic Medical Center, Amsterdam, The Netherlands; Laboratory of Experimental Intensive Care and Anesthesiology, Academic Medical Center, Amsterdam, The Netherlands.
Laboratory of Experimental Clinical Chemistry, Academic Medical Center, Amsterdam, The Netherlands.
出版信息
Transfus Med Hemother. 2016 Jul;43(4):302-305. doi: 10.1159/000442681. Epub 2015 Dec 16.
BACKGROUND
Transfusion of red blood cells (RBCs) is associated with adverse outcome, but the causative factor is unknown. Extracellular vesicles (EVs) have pro-inflammatory properties. We hypothesized that EVs released from both fresh and stored RBC products can induce a host inflammatory response in a dose-dependent manner.
METHODS
Whole blood was incubated with supernatant from RBC units stored for different time periods, either containing (different numbers of) EVs or depleted from EVs.
RESULTS
Incubation with both fresh and stored supernatant containing EVs induced a strong host response with production of TNF, IL-6 and IL-8. In supernatant depleted from EVs, this host response was completely abrogated. IL-10 levels were not affected. EV-induced host response was both dependent on the number of EVs as well as on storage time.
CONCLUSIONS
EVs from both fresh and stored RBC units illicit a strong inflammatory host response in recipients and may therefore contribute to adverse outcome of RBC transfusion.
背景
输注红细胞（RBC）与不良后果相关，但致病因素尚不清楚。细胞外囊泡（EVs）具有促炎特性。我们假设新鲜和储存的RBC制品释放的EVs可诱导宿主产生剂量依赖性炎症反应。
方法
将全血与储存不同时间段的RBC单位上清液孵育，上清液含有（不同数量的）EVs或不含EVs。
结果
与含有EVs的新鲜和储存上清液孵育均诱导宿主产生强烈反应，产生肿瘤坏死因子（TNF）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）。在不含EVs的上清液中，这种宿主反应完全消除。白细胞介素-10（IL-10）水平不受影响。EV诱导的宿主反应既取决于EV的数量，也取决于储存时间。
结论
新鲜和储存的RBC单位中的EVs在受者中引发强烈的炎症宿主反应，因此可能导致RBC输血的不良后果。
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