Palcic B, Skarsgard L D
Br J Cancer Suppl. 1978 Jun;3:54-9.
The loss of colony-forming ability and the yield of DNA single strand breaks were studied following exposure of various mammalian cell types to misonidazole. A correlation was observed between cell inactivation and DNA damage. If, after exposure, the cells were washed free of the drug and then incubated at 37 degree C in growth medium, it was observed that surviving cells repaired their DNA to the point that sedimentation profiles were identical to those of unexposed cells. In nonsurviving cells, however, the DNA was found to be further degraded with post-exposure incubation.
在将各种哺乳动物细胞类型暴露于米索硝唑后，研究了其集落形成能力的丧失和DNA单链断裂的产量。观察到细胞失活与DNA损伤之间存在相关性。如果在暴露后将细胞冲洗以去除药物，然后在37℃的生长培养基中孵育，则观察到存活细胞将其DNA修复到沉降图谱与未暴露细胞相同的程度。然而，在未存活的细胞中，发现DNA在暴露后孵育时会进一步降解。
