Cozer Aline G, Trapp Márcia, Vieira Marques Claudia, Martins Tiago L, Model Jorge Felipe A, Schein Vanessa, Kucharski Luiz Carlos, Da Silva Roselis S M
Department of Physiology, Institute of Health Sciences, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil.
Lipids. 2016 Nov;51(11):1303-1307. doi: 10.1007/s11745-016-4202-5. Epub 2016 Oct 11.
The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the incorporation of C from glucose into lipids in the rat WRAT. The increase in lipogenesis capacity supports the hypothesis that the activity of the glycerol-3-phosphate-generating pathway (glycolysis) from glucose is regulated by hSTC-1. The effect of hSTC-1 on de novo fatty acid synthesis and on glucose oxidation in WRAT is supported by an 85 % increase in CO production from C-glucose. The incubation of WRAT in the presence of hSTC-1 maintained the ADP/ATP ratio close to the control group. The presence of hSTC-1 in the incubation medium did not inhibit the lipolytic effect of epinephrine. In conclusion, hSTC-1 is one of the hormonal factors that control glucose metabolism in WRAT in the fed state.
本研究在体外评估了人鲽源钙调蛋白1（hSTC-1）在喂食大鼠的腹膜后白色脂肪组织（WRAT）葡萄糖代谢中的作用。在喂食状态下，hSTC1增加了大鼠WRAT中葡萄糖的碳掺入脂质的量。脂肪生成能力的增加支持了以下假设：来自葡萄糖的甘油-3-磷酸生成途径（糖酵解）的活性受hSTC-1调节。WRAT中从头脂肪酸合成和葡萄糖氧化的hSTC-1效应得到了来自碳葡萄糖的CO产生增加85%的支持。在hSTC-1存在下孵育WRAT可使ADP/ATP比值维持在接近对照组的水平。孵育培养基中存在hSTC-1不会抑制肾上腺素的脂解作用。总之，hSTC-1是在喂食状态下控制WRAT葡萄糖代谢的激素因子之一。
