CCMAR/CIMAR - Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
AWI - Integrative Ecophysiology, Alfred-Wegener-Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570, Bremerhaven, Germany.
Sci Rep. 2019 Jan 24;9(1):723. doi: 10.1038/s41598-018-36821-2.
Stanniocalcin 1 (STC1) and parathyroid hormone-related protein (PTHrP) are calciotropic hormones in vertebrates. Here, a recently hypothesized metabolic role for these hormones is tested on European sea bass treated with: (i) teleost PTHrP(1-34), (ii) PTHrP(1-34) and anti-STC1 serum (pro-PTHrP groups), (iii) a PTHrP antagonist PTHrP(7-34) or (iv) PTHrP(7-34) and STC1 (pro-STC1 groups). Livers were analysed using untargeted metabolic profiling based on proton nuclear magnetic resonance (H-NMR) spectroscopy. Concentrations of branched-chain amino acid (BCAA), alanine, glutamine and glutamate increased in pro-STC1 groups suggesting their mobilization from the muscle to the liver for degradation and gluconeogenesis from alanine and glutamine. In addition, only STC1 treatment decreased the concentrations of succinate, fumarate and acetate, indicating slowing of the citric acid cycle. In the pro-PTHrP groups the concentrations of glucose, erythritol and lactate decreased, indicative of gluconeogenesis from lactate. Taurine, trimethylamine, trimethylamine N-oxide and carnitine changed in opposite directions in the pro-STC1 versus the pro-PTHrP groups, suggesting opposite effects, with STC1 stimulating lipogenesis and PTHrP activating lipolysis/β-oxidation of fatty acids. These findings suggest a role for STC1 and PTHrP related to strategic energy mechanisms that involve the production of glucose and safeguard of liver glycogen reserves for stressful situations.
硬骨鱼钙调激素 1（STC1）和甲状旁腺激素相关蛋白（PTHrP）是脊椎动物中的钙调激素。本研究以欧洲鲈鱼为模型，检测了这些激素的一种新假说的代谢作用：（i）硬骨鱼 PTHrP（1-34），（ii）PTHrP（1-34）和抗 STC1 血清（pro-PTHrP 组），（iii）PTHrP 拮抗剂 PTHrP（7-34）或（iv）PTHrP（7-34）和 STC1（pro-STC1 组）处理。采用基于质子核磁共振（H-NMR）光谱的非靶向代谢组学分析肝脏。pro-STC1 组中支链氨基酸（BCAA）、丙氨酸、谷氨酰胺和谷氨酸浓度增加，表明它们从肌肉动员到肝脏进行降解，丙氨酸和谷氨酰胺进行糖异生。此外，只有 STC1 处理降低了琥珀酸、富马酸和乙酸盐的浓度，表明柠檬酸循环减缓。在 pro-PTHrP 组中，葡萄糖、赤藓糖醇和乳酸盐的浓度降低，表明从乳酸盐进行糖异生。牛磺酸、三甲胺、三甲胺氧化物和肉碱在 pro-STC1 与 pro-PTHrP 组中的变化方向相反，表明作用相反，STC1 刺激脂肪生成，PTHrP 激活脂肪酸的脂肪分解/β-氧化。这些发现表明 STC1 和 PTHrP 与涉及葡萄糖产生和保护肝脏糖原储备以应对应激情况的战略能量机制有关。
