Załuski Daniel, Smolarz Helena D
Department of Pharmacognosy, Collegium Medicum, Jagiellonian University, Cracow, Poland / Faculty of Health Sciences, Almamer University, Warsaw, Poland.
Department of Pharmaceutical Botany, Medical University of Lublin, Poland.
Pak J Pharm Sci. 2016 Sep;29(5):1497-1502.
The chemical composition and pharmacological activity of E. senticosus cultivated in Poland were investigated. Studies included the assay of TPC and TFC, 2D-TLC identification of phenolic acids, HPTLC-detection of antioxidants, and antioxidative, antileukemic, anti-MMPs properties of E. senticosus. The ethanolic extracts from the roots, spring leaves, fruits, and the chloroform extract from the roots were tested. The richest in polyphenols are the fresh fruits (57.5 mg/g), while in flavonoids the spring leaves (27.4 mg/g). The antioxidant ability both in extracts and single phenolic constituents were checked out by the measurement of the DPPH radical scavenging activity, iron (II) chelating and lipid peroxidation inhibitory activity. Using HPTLC-DB test eleutherosides B and E1 have been found as the phenolic antioxidants. Thirty six percent of apoptotic cells have been observed in Jurkatt 45 line by the treatment with the chloroform extract from the roots. Only the chloroform extract from the roots and the ethanolic one from the dried fruits have shown the inhibitory activities against MMPs. It is noteworthy, that our studies have been done for the first time, and the plant material has come from another geographical zone (Poland) than native (Asia).
对波兰种植的刺五加的化学成分和药理活性进行了研究。研究包括总酚含量（TPC）和总黄酮含量（TFC）的测定、酚酸的二维薄层色谱（2D-TLC）鉴定、高效薄层色谱（HPTLC）对抗氧化剂的检测以及刺五加的抗氧化、抗白血病、抗基质金属蛋白酶（MMPs）特性。对根、春季叶片、果实的乙醇提取物以及根的氯仿提取物进行了测试。多酚含量最丰富的是新鲜果实（57.5毫克/克），而黄酮类含量最高的是春季叶片（27.4毫克/克）。通过测定二苯基苦味酰基自由基（DPPH）清除活性、铁（II）螯合和脂质过氧化抑制活性，对提取物和单一酚类成分的抗氧化能力进行了检测。使用HPTLC-DB测试发现刺五加苷B和E1是酚类抗氧化剂。用根的氯仿提取物处理Jurkatt 45细胞系后，观察到36%的凋亡细胞。只有根的氯仿提取物和干果的乙醇提取物显示出对MMPs 的抑制活性。值得注意的是，我们的研究是首次进行，并且植物材料来自与原生地（亚洲）不同的地理区域（波兰）。
