Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Marie Curie-Skłodowska 9, 85-094 Bydgoszcz, Poland.
Department of Pharmacology, Faculty of Health Sciences, Medical University of Lublin, 4a Chodzki Str., 20-093 Lublin, Poland.
Molecules. 2022 Aug 30;27(17):5579. doi: 10.3390/molecules27175579.
(Rupr. et Maxim.) Maxim. is well-known for its adaptogenic properties in traditional Eastern medicine. It has been categorized as an endangered species due to the over-exploitation of the roots. As a result, alternatives must be found, including the usage of renewable aerial parts such as fruits. The goal of this research was to determine the phenolic compounds and the enzymatic, antioxidant, and cytotoxic activities of the intractum gained from the fruits and the mixture of chloroform-methanol roots extract with naringenin (3:7:5). The obtained results showed, that the intractum contained 1.02 mg/g ext. of polyphenols, 0.30 mg/g ext. of flavonoids, and 0.19 mg/g ext. of phenolic acids. In turn, the mixture of chloroform-methanol roots extract with naringenin (3:7:5) contained 159.27 mg/g ext. of polyphenols, 137.47 mg/g ext. of flavonoids, and 79.99 mg/g ext. of phenolic acids. Regarding the anti-enzymatic assay, the IC values for tyrosinase and hyaluronidase were equal to 586.83 and 217.44 [μg/mL] for the intractum, and 162.56 and 44.80 [μg/mL] for the mixture, respectively. Both preparations have possessed significant antioxidant activity in the ABTS, DPPH, and ferrozine tests. No cytotoxic effect on the FaDu and HEP G2 cancer cell lines was observed. Our findings support the traditional use of fruits and roots. Moreover, the results indicate also that adaptogens are rather nontoxic for normal and cancer cells, which corresponds with some hypotheses on adaptogens activity.
（Rupr. et Maxim.）Maxim. 以其在传统东方医学中的适应原特性而闻名。由于根部的过度开采，它已被归类为濒危物种。因此，必须找到替代品，包括使用可再生的地上部分，如果实。本研究的目的是确定从果实和氯仿-甲醇根提取物与柚皮素（3:7:5）的混合物中获得的浸膏中的酚类化合物以及酶、抗氧化和细胞毒性活性。研究结果表明，浸膏中含有 1.02mg/g 提取物的多酚、0.30mg/g 提取物的类黄酮和 0.19mg/g 提取物的酚酸。相反，氯仿-甲醇根提取物与柚皮素（3:7:5）的混合物中含有 159.27mg/g 提取物的多酚、137.47mg/g 提取物的类黄酮和 79.99mg/g 提取物的酚酸。关于抗酶测定，酪氨酸酶和透明质酸酶的 IC 值分别为浸膏的 586.83 和 217.44[μg/mL]，混合物的 162.56 和 44.80[μg/mL]。两种制剂在 ABTS、DPPH 和 ferrozine 试验中均具有显著的抗氧化活性。在 FaDu 和 HEP G2 癌细胞系中未观察到细胞毒性作用。我们的研究结果支持果实和根的传统用途。此外，结果还表明适应原对正常细胞和癌细胞的毒性较小，这与适应原活性的一些假说相符。
