Liller Corinna B, Walla Agatha, Boer Martin P, Hedley Pete, Macaulay Malcolm, Effgen Sieglinde, von Korff Maria, van Esse G Wilma, Koornneef Maarten
Department Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany.
Institute of Plant Genetics, Heinrich Heine University Duesseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany.
Theor Appl Genet. 2017 Feb;130(2):269-281. doi: 10.1007/s00122-016-2807-y. Epub 2016 Oct 12.
Awn length was mapped using a multiparent population derived from cv. Morex and four wild accessions. One QTL was fine mapped and candidate genes were identified in NILs by RNA-seq. Barley awns are photosynthetically active and contribute to grain yield. Awn length is variable among both wild and cultivated barley genotypes and many mutants with alterations in awn length have been identified. Here, we used a multiparent mapping population derived from cv. Morex and four genetically diverse wild barley lines to detect quantitative trait loci (QTLs) for awn length. Twelve QTLs, distributed over the barley genome, were identified with the most significant one located on chromosome arm 7HL (QTL AL7.1). The effect of AL7.1 was confirmed using near isogenic lines (NILs) and fine-mapped in two independent heterogeneous inbred families to a < 0.9 cM interval. With exception of a small effect on grain width, no other traits such as plant height or flowering time were affected by AL7.1. Variant calling on transcripts obtained from RNA sequencing reads in NILs was used to narrow down the list of candidate genes located in the interval. This data may be used for further characterization and unravelling of the mechanisms underlying natural variation in awn length.
使用源自栽培品种Morex和四个野生材料的多亲本群体对芒长进行定位。通过RNA测序在近等基因系中精细定位了一个QTL并鉴定了候选基因。大麦芒具有光合活性并对籽粒产量有贡献。芒长在野生和栽培大麦基因型中均存在变异，并且已经鉴定出许多芒长发生改变的突变体。在此，我们使用源自栽培品种Morex和四个遗传多样的野生大麦品系的多亲本定位群体来检测芒长的数量性状位点（QTL）。在大麦基因组上共鉴定出12个QTL，其中最显著的一个位于染色体臂7HL上（QTL AL7.1）。利用近等基因系（NIL）证实了AL7.1的效应，并在两个独立的异质自交家系中将其精细定位到小于0.9 cM的区间。除了对籽粒宽度有较小影响外，AL7.1对株高或开花时间等其他性状没有影响。利用从NIL的RNA测序读数获得的转录本进行变异检测，以缩小位于该区间的候选基因列表。这些数据可用于进一步表征和揭示芒长自然变异的潜在机制。
