Guo Huiqin, Xiong Jingjing, Ma Wentian, Wu Minghuo, Yan Liushui, Li Kexin, Liu Yu
Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle, School of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang, P. R. China.
School of Food and Environmental Science and Technology, Dalian University of Technology, Panjin, P. R. China.
J Sep Sci. 2016 Nov;39(22):4439-4448. doi: 10.1002/jssc.201600834. Epub 2016 Nov 2.
Molecularly imprinted polymers were synthesized using mixed tea saponins as a template and acrylamide-β-cyclodextrin as a cofunctional monomer for the specific binding and purification of tea saponins from the defatted cake extract of Camellia oleifera. The adsorption properties of the prepared polymers were systematically evaluated including adsorption kinetics, adsorption isotherms, and selective recognition characteristics. It showed that the adsorption kinetics followed the pseudo first-order kinetic model (R = 0.995) with an equilibrium time of 3 h, adsorption isotherm data fitted well with the Langmuir-Freundlich model (R = 0.984) with an adsorption capacity of 14.23 mg/g. The relative selectivity coefficient (k´) in the presence of the analogues glycyrrhizic acid and glycyrrhetinic acid were 1.16 and 17.21, respectively. The performance of the molecularly imprinted polymers as solid-phase extraction materials was investigated and the results indicated that using acrylamide-β-cyclodextrin as a cofunctional monomer improved both the adsorption capacity and active sites stability of the imprinted polymers. The solid-phase extraction using the polymers as packing materials was subsequently applied for the separation of tea saponins in raw C. oleifera press extract, and targets were obtained with a purity reaching 89%.
以混合茶皂素为模板，丙烯酰胺-β-环糊精为共功能单体，合成了分子印迹聚合物，用于从油茶脱脂饼提取物中特异性结合和纯化茶皂素。系统评价了所制备聚合物的吸附性能，包括吸附动力学、吸附等温线和选择性识别特性。结果表明，吸附动力学符合准一级动力学模型（R = 0.995），平衡时间为3 h，吸附等温线数据与Langmuir-Freundlich模型拟合良好（R = 0.984），吸附容量为14.23 mg/g。在甘草酸和甘草次酸类似物存在下的相对选择性系数（k´）分别为1.16和17.21。研究了分子印迹聚合物作为固相萃取材料的性能，结果表明，使用丙烯酰胺-β-环糊精作为共功能单体提高了印迹聚合物的吸附容量和活性位点稳定性。随后将以该聚合物为填充材料的固相萃取应用于油茶粗榨提取物中茶皂素的分离，得到纯度达89%的目标产物。
