Håskjold E, Bjerknes R, Bjerknes E
Institute of Pathology, University of Oslo, Rikshospitalet, Norway.
Acta Ophthalmol (Copenh). 1989 Feb;67(1):91-6. doi: 10.1111/j.1755-3768.1989.tb00730.x.
The migration of cells in the rat corneal epithelium was studied using continuous labelling with tritiated thymidine [3H]TdR, during a 24 h period. Most mitoses resulted in 2 new basal cells. Cells leaving the basal cell layer moved vertically to the surface in all areas of the corneal epithelium. The first labelled cells reached the surface 3 days after the first injection of [3H]TdR. No stream of cells from the conjunctiva to the cornea in the limbal areas was observed, and no centripetal migration of epithelial cells in the cornea could be observed. After a cell has lost its attachment to the basement membrane, it is committed to be exfoliated in a few days without undergoing mitosis. Thus the slow centripetal migration of epithelial cells and the exchange of centrally located cells, as indicated by clinical findings and experimental studies, can only be explained by migration of basal cells.
在24小时内，使用氚标记胸腺嘧啶核苷[3H]TdR连续标记法研究大鼠角膜上皮细胞的迁移。大多数有丝分裂产生2个新的基底细胞。离开基底细胞层的细胞在角膜上皮的所有区域垂直向表面移动。首次注射[3H]TdR后3天，首批标记细胞到达表面。在角膜缘区域未观察到从结膜到角膜的细胞流，也未观察到角膜上皮细胞的向心迁移。细胞与基底膜失去附着后，会在几天内脱落而不进行有丝分裂。因此，临床发现和实验研究表明的上皮细胞缓慢向心迁移和中央位置细胞的交换，只能通过基底细胞的迁移来解释。
